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Method Article
Parallel DNA Polymerase Chain Reaction (PD-PCR)
Vikash bhardwaj
Molecular Biology and Genetics Domain, Lovely Professional University, Punjab, 144411, India
Corresponding Author
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This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describe an approach in which the first primer binds in a parallel complementary orientation to the single-stranded DNA, leading to synthesis in a parallel direction. Further reactions happened in a conventional way leading to the synthesis of PCR product having polarity opposite to the template used.
Keywords
PCR, PD-PCR, DNA synthesis
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Associated Publications
Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template
by Vikash Bhardwaj and Kulbhushan Sharma
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This is a preprint. It has not completed peer review.
Method Article
Parallel DNA Polymerase Chain Reaction (PD-PCR)
Vikash bhardwaj
Molecular Biology and Genetics Domain, Lovely Professional University, Punjab, 144411, India
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 05 May, 2016
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Molecular Biology
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describe an approach in which the first primer binds in a parallel complementary orientation to the single-stranded DNA, leading to synthesis in a parallel direction. Further reactions happened in a conventional way leading to the synthesis of PCR product having polarity opposite to the template used.
Figure 1
Figure 2
Associated Publications
Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template
by Vikash Bhardwaj and Kulbhushan Sharma