An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Vikash bhardwaj
Molecular Biology and Genetics Domain, Lovely Professional University, Punjab, 144411, India
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describe an approach in which the first primer binds in a parallel complementary orientation to the single-stranded DNA, leading to synthesis in a parallel direction. Further reactions happened in a conventional way leading to the synthesis of PCR product having polarity opposite to the template used.
Keywords
PCR, PD-PCR, DNA synthesis
Figure 1
Figure 2
No competing interests were disclosed.
Loading...
Version History
CURRENT STATUS: Posted
Version 1
Posted 05 May, 2016
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Molecular Biology
Associated Publications
Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template
by Vikash Bhardwaj and Kulbhushan Sharma
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Vikash bhardwaj
Molecular Biology and Genetics Domain, Lovely Professional University, Punjab, 144411, India
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 05 May, 2016
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Molecular Biology
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describe an approach in which the first primer binds in a parallel complementary orientation to the single-stranded DNA, leading to synthesis in a parallel direction. Further reactions happened in a conventional way leading to the synthesis of PCR product having polarity opposite to the template used.
Figure 1
Figure 2
No competing interests were disclosed.
Loading...
Associated Publications
Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template
by Vikash Bhardwaj and Kulbhushan Sharma