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Method Article
Magdalena Zernicka-Goetz
Zernicka-Goetz's lab, University of Cambridge
Corresponding Author
License:
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The development of the human embryo beyond implantation has been poorly characterised, because in vivo experiments are unfeasible and there is no appropriate in vitro culture system. Here, we describe a protocol to culture human embryos from pre- to post-implantation stages in vitro, in the absence of maternal tissues. This protocol represents a unique opportunity to study the cellular and molecular mechanisms of human embryo development beyond implantation. In addition, we report a protocol to recapitulate polarisation and lumenogenesis of the embryonic lineage using human pluripotent stem cell lines (hPSCs). We anticipate that future studies using these two in vitro culture systems will help us understand how embryonic and extra-embryonic tissues cooperate to generate the basic human body plan.
Keywords
Human embryo, pluripotent stem cells, morphogenesis
The authors declare no competing financial interests
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Associated Publications
Self-organization of the human embryo in the absence of maternal tissues
by Marta N. Shahbazi, Agnieszka Jedrusik, Sanna Vuoristo, +11
Nature Cell Biology (05 May, 2016)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Magdalena Zernicka-Goetz
Zernicka-Goetz's lab, University of Cambridge
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 05 May, 2016
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The development of the human embryo beyond implantation has been poorly characterised, because in vivo experiments are unfeasible and there is no appropriate in vitro culture system. Here, we describe a protocol to culture human embryos from pre- to post-implantation stages in vitro, in the absence of maternal tissues. This protocol represents a unique opportunity to study the cellular and molecular mechanisms of human embryo development beyond implantation. In addition, we report a protocol to recapitulate polarisation and lumenogenesis of the embryonic lineage using human pluripotent stem cell lines (hPSCs). We anticipate that future studies using these two in vitro culture systems will help us understand how embryonic and extra-embryonic tissues cooperate to generate the basic human body plan.
The authors declare no competing financial interests
Loading...
Associated Publications
Self-organization of the human embryo in the absence of maternal tissues
by Marta N. Shahbazi, Agnieszka Jedrusik, Sanna Vuoristo, +11
Nature Cell Biology (05 May, 2016)