An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Henning Gram Hansen
CHO Cell Line Engineering and Design, NNF-CfB, Technical University of Denmark
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Accurate assays for counting mammalian cells are important tools when performing experiments in cell biology. In general, commercially available cell-counting assays suffer from low throughput and high costs of consumables. Here, we describe a cost-efficient Hoechst and propidium iodide (PI)-staining protocol that accurately counts the number of viable mammalian cells in 96-well microplates based on image cytometry on a Celígo® Image Cytometer. Hands-on time for one microplate is less than 20 minutes and the entire procedure including incubation takes approximately 1 hour. Since all steps prior to image cytometry analysis involve liquid handling and since the Celígo® Image Cytometer is automation-friendly, this protocol has the potential to be integrated into automated liquid handling workflows.
Keywords
VCD, viability, mammalian cells, CHO, high-throughput, Celigo
Figure 1
I declare no conflict of interest.
Loading...
Version History
CURRENT STATUS: Posted
Version 1
Posted 20 Jun, 2016
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Associated Publications
Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells
by Henning Gram Hansen, Claes Nymand Nilsson, Anne Mathilde Lund, +7
Scientific Reports (21 March, 2016)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Henning Gram Hansen
CHO Cell Line Engineering and Design, NNF-CfB, Technical University of Denmark
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 20 Jun, 2016
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Accurate assays for counting mammalian cells are important tools when performing experiments in cell biology. In general, commercially available cell-counting assays suffer from low throughput and high costs of consumables. Here, we describe a cost-efficient Hoechst and propidium iodide (PI)-staining protocol that accurately counts the number of viable mammalian cells in 96-well microplates based on image cytometry on a Celígo® Image Cytometer. Hands-on time for one microplate is less than 20 minutes and the entire procedure including incubation takes approximately 1 hour. Since all steps prior to image cytometry analysis involve liquid handling and since the Celígo® Image Cytometer is automation-friendly, this protocol has the potential to be integrated into automated liquid handling workflows.
Figure 1
I declare no conflict of interest.
Loading...
Associated Publications
Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells
by Henning Gram Hansen, Claes Nymand Nilsson, Anne Mathilde Lund, +7
Scientific Reports (21 March, 2016)