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Method Article
Dan Gazit
Gazit Lab
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Osteoporosis-related fractures are becoming more common as the world population ages, and they constitute an unmet clinical need. Animal models are essential for the preclinical development of novel therapeutic strategies, such as stem cells, for osteoporosis-related fractures. While a number of animal models exist, this protocol describes a highly reproducible method of generating multiple bone defects in young osteoporotic immunodeficient rats. In addition, we describe a novel longitudinal, semiautomated, micro–computed tomography-based quantitative structural analysis of bone regeneration. This imaging approach can be adapted for a variety of other bone defects models in which longitudinal imaging–based analyses could benefit from precise 3D semiautomated alignment. Taken together, this protocol describes a readily quantifiable and easily reproducible system for osteoporosis and bone research. The suggested protocol takes 4 months to induce osteoporosis and 2.7 to 4 hours to generate, image, and analyze two vertebral defects, depending on tissue size and equipment.
Keywords
osteoporosis, vertebral fracture, rodent model, micro–computed tomography
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The authors declare no competing financial interests.
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CURRENT STATUS: Posted
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Posted 01 Apr, 2016
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Biological techniques
Associated Publications
PTH Induces Systemically Administered Mesenchymal Stem Cells to Migrate to and Regenerate Spine Injuries
by Dmitriy Sheyn, Galina Shapiro, Wafa Tawackoli, +16
Molecular Therapy (16 March, 2016)
Method Article
Dan Gazit
Gazit Lab
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 01 Apr, 2016
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Osteoporosis-related fractures are becoming more common as the world population ages, and they constitute an unmet clinical need. Animal models are essential for the preclinical development of novel therapeutic strategies, such as stem cells, for osteoporosis-related fractures. While a number of animal models exist, this protocol describes a highly reproducible method of generating multiple bone defects in young osteoporotic immunodeficient rats. In addition, we describe a novel longitudinal, semiautomated, micro–computed tomography-based quantitative structural analysis of bone regeneration. This imaging approach can be adapted for a variety of other bone defects models in which longitudinal imaging–based analyses could benefit from precise 3D semiautomated alignment. Taken together, this protocol describes a readily quantifiable and easily reproducible system for osteoporosis and bone research. The suggested protocol takes 4 months to induce osteoporosis and 2.7 to 4 hours to generate, image, and analyze two vertebral defects, depending on tissue size and equipment.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Loading...
Associated Publications
PTH Induces Systemically Administered Mesenchymal Stem Cells to Migrate to and Regenerate Spine Injuries
by Dmitriy Sheyn, Galina Shapiro, Wafa Tawackoli, +16
Molecular Therapy (16 March, 2016)