Semiautomated longitudinal micro–computed tomography–based quantitative structural analysis of bone regeneration in osteoporotic rats
Osteoporosis-related fractures are becoming more common as the world population ages, and they constitute an unmet clinical need. Animal models are essential for the preclinical development of novel therapeutic strategies, such as stem cells, for osteoporosis-related fractures. While a number of animal models exist, this protocol describes a highly reproducible method of generating multiple bone defects in young osteoporotic immunodeficient rats. In addition, we describe a novel longitudinal, semiautomated, micro–computed tomography-based quantitative structural analysis of bone regeneration. This imaging approach can be adapted for a variety of other bone defects models in which longitudinal imaging–based analyses could benefit from precise 3D semiautomated alignment. Taken together, this protocol describes a readily quantifiable and easily reproducible system for osteoporosis and bone research. The suggested protocol takes 4 months to induce osteoporosis and 2.7 to 4 hours to generate, image, and analyze two vertebral defects, depending on tissue size and equipment.
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Posted 01 Apr, 2016
Semiautomated longitudinal micro–computed tomography–based quantitative structural analysis of bone regeneration in osteoporotic rats
Posted 01 Apr, 2016
Osteoporosis-related fractures are becoming more common as the world population ages, and they constitute an unmet clinical need. Animal models are essential for the preclinical development of novel therapeutic strategies, such as stem cells, for osteoporosis-related fractures. While a number of animal models exist, this protocol describes a highly reproducible method of generating multiple bone defects in young osteoporotic immunodeficient rats. In addition, we describe a novel longitudinal, semiautomated, micro–computed tomography-based quantitative structural analysis of bone regeneration. This imaging approach can be adapted for a variety of other bone defects models in which longitudinal imaging–based analyses could benefit from precise 3D semiautomated alignment. Taken together, this protocol describes a readily quantifiable and easily reproducible system for osteoporosis and bone research. The suggested protocol takes 4 months to induce osteoporosis and 2.7 to 4 hours to generate, image, and analyze two vertebral defects, depending on tissue size and equipment.
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