Heart ink injection

doi:10.1038/nprot.2008.161

Method Article

Heart ink injection

https://doi.org/10.1038/nprot.2008.161

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Cell biology

Developmental biology

Biological techniques

heart

ink injection

blood vessel

With this technique it is possible to label the blood vessel in vivo in order to analyze in whole mount the quantity and quality of blood vessel.

1.0 ml syringe

China Black Ink

Stadard anesthetic (Ketamine and Xylazine)

Anesthetize the mouse
Using a 1 ml syringe withdraw 0,5 ml of mouse blood from the heart ventricle
Inject 1 ml of China Ink and wait for 30 sec (until the eyes, mouth and legs became black)
Quickly dissect and fix in 4% PFA over night at 4°C
Treat the sample with a solution of 0,5% KOH for one day
Treat the sample with a solution of 0,5% KOH/Glycarol 2:1 one day
Treat the sample with a solution of 0,5% KOH/Glycerol 1:1 for one day
Treat the sample with 100% Glycerol for one day (in order to visualize clearly all the inner blood vessels)

4 days

Injection in the heart after blood withdrawal

eyes, mouth and legs black stained

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Heart ink injection

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Heart ink injection

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Figures

Introduction

Reagents

Procedure

Timing

Critical Steps

Anticipated Results

Associated Publications

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Terms of Service

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