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Method Article
Claudio Ando Nicolini
Claudio Ando Nicolini's Lab, NanoWorld, USA; HC Professor Nanobiotechnology, Lomonosov Moscow State University, Russia; Foreign Member Russian Academy of Sciences; President NWI Fondazione EL.B.A. Nicolini; Editor-in-Chief NWJ, Santa Clara, USA
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
In the current protocol, we explain how to perform label-free mass spectrometry (MS)-based analysis of gene expression and proteins. We coupled Matrix-assisted laser desorption ionization (MALDI) Time of flight (TOF) with cell-free in vitro transcription and translation (IVTT) technologies, namely Nucleic Acid Protein Programmable Array (NAPPA) and SNAP techniques. Data analysis is performed by the usage of SpADS, an R package that takes advantage of simple data mining algorithms.
The SpADS package is freely available at "http://www.ibf.unige.it/SpADS":http://www.ibf.unige.it/SpADS
Keywords
label-free technologies, mass spectrometry, NAPPA, SNAP, cancer
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The authors declare no competing financial interests.
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Associated Publications
MALDI-TOF characterization of NAPPA-generated proteins
by Rosanna Spera, Joshua Labaer, and Claudio Nicolini
Journal Of Mass Spectrometry (25 January, 2016)
10.1016/B978-0-12-800453-1.00004-X
Method Article
Claudio Ando Nicolini
Claudio Ando Nicolini's Lab, NanoWorld, USA; HC Professor Nanobiotechnology, Lomonosov Moscow State University, Russia; Foreign Member Russian Academy of Sciences; President NWI Fondazione EL.B.A. Nicolini; Editor-in-Chief NWJ, Santa Clara, USA
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 04 Feb, 2016
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
In the current protocol, we explain how to perform label-free mass spectrometry (MS)-based analysis of gene expression and proteins. We coupled Matrix-assisted laser desorption ionization (MALDI) Time of flight (TOF) with cell-free in vitro transcription and translation (IVTT) technologies, namely Nucleic Acid Protein Programmable Array (NAPPA) and SNAP techniques. Data analysis is performed by the usage of SpADS, an R package that takes advantage of simple data mining algorithms.
The SpADS package is freely available at "http://www.ibf.unige.it/SpADS":http://www.ibf.unige.it/SpADS
Figure 1
Figure 2
Figure 3
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Associated Publications
MALDI-TOF characterization of NAPPA-generated proteins
by Rosanna Spera, Joshua Labaer, and Claudio Nicolini
Journal Of Mass Spectrometry (25 January, 2016)
10.1016/B978-0-12-800453-1.00004-X