Label-free Mass-Spectrometry (MS) for Cancer control Protocol
In the current protocol, we explain how to perform label-free mass spectrometry (MS)-based analysis of gene expression and proteins. We coupled Matrix-assisted laser desorption ionization (MALDI) Time of flight (TOF) with cell-free in vitro transcription and translation (IVTT) technologies, namely Nucleic Acid Protein Programmable Array (NAPPA) and SNAP techniques. Data analysis is performed by the usage of SpADS, an R package that takes advantage of simple data mining algorithms.
The SpADS package is freely available at "http://www.ibf.unige.it/SpADS":http://www.ibf.unige.it/SpADS
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Posted 04 Feb, 2016
Label-free Mass-Spectrometry (MS) for Cancer control Protocol
Posted 04 Feb, 2016
In the current protocol, we explain how to perform label-free mass spectrometry (MS)-based analysis of gene expression and proteins. We coupled Matrix-assisted laser desorption ionization (MALDI) Time of flight (TOF) with cell-free in vitro transcription and translation (IVTT) technologies, namely Nucleic Acid Protein Programmable Array (NAPPA) and SNAP techniques. Data analysis is performed by the usage of SpADS, an R package that takes advantage of simple data mining algorithms.
The SpADS package is freely available at "http://www.ibf.unige.it/SpADS":http://www.ibf.unige.it/SpADS
Figure 1
Figure 2
Figure 3
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