Time kill assays for Streptococcus agalactiae and synergy testing
Group B Streptococcus (GBS) is increasingly causing invasive infections in nonpregnant adults. Previously a synergism in killing the bacteria was postulated, when a combination of penicillin plus gentamicin is used. This synergism is based on results from time-kill assays. Although, the technique of time-kill assays is known for decades, several variables may influence the stability of results, and vary for a given bacterial genus and its species. These include the incubation volume, the media, the proportion of CO2, and the use of a shaker. In this protocol, we compared these conditions with each other and outline the test results of the corresponding time-kill assays.
I need a clarification regarding result reading. when we have converted the cfu/ml into log values , do we have to find the difference of the log cfu/ml of the antibiotics with the log values of the starting inoculum? the answer would be appreciated and valued. thank you
Posted 28 Dec, 2015
Time kill assays for Streptococcus agalactiae and synergy testing
Posted 28 Dec, 2015
Group B Streptococcus (GBS) is increasingly causing invasive infections in nonpregnant adults. Previously a synergism in killing the bacteria was postulated, when a combination of penicillin plus gentamicin is used. This synergism is based on results from time-kill assays. Although, the technique of time-kill assays is known for decades, several variables may influence the stability of results, and vary for a given bacterial genus and its species. These include the incubation volume, the media, the proportion of CO2, and the use of a shaker. In this protocol, we compared these conditions with each other and outline the test results of the corresponding time-kill assays.
I need a clarification regarding result reading. when we have converted the cfu/ml into log values , do we have to find the difference of the log cfu/ml of the antibiotics with the log values of the starting inoculum? the answer would be appreciated and valued. thank you
© Research Square 2021