This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
Time kill assays for Streptococcus agalactiae and synergy testing
Parham Sendi
Institute for Infectious Diseases, University of Bern, Switzerland
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Group B Streptococcus (GBS) is increasingly causing invasive infections in nonpregnant adults. Previously a synergism in killing the bacteria was postulated, when a combination of penicillin plus gentamicin is used. This synergism is based on results from time-kill assays. Although, the technique of time-kill assays is known for decades, several variables may influence the stability of results, and vary for a given bacterial genus and its species. These include the incubation volume, the media, the proportion of CO2, and the use of a shaker. In this protocol, we compared these conditions with each other and outline the test results of the corresponding time-kill assays.
Keywords
Time kill assays, Synergism, Group B Streptococcus
sowmya TN
commented on 26 April, 2020
I need a clarification regarding result reading. when we have converted the cfu/ml into log values , do we have to find the difference of the log cfu/ml of the antibiotics with the log values of the starting inoculum? the answer would be appreciated and valued. thank you
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 28 Dec, 2015
Community comments: 1
Metrics
Comments: 1
HTML Views: ...
Subject Areas
Microbiology
Learn more about our company.
This is a preprint. It has not completed peer review.
Method Article
Time kill assays for Streptococcus agalactiae and synergy testing
Parham Sendi
Institute for Infectious Diseases, University of Bern, Switzerland
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 28 Dec, 2015
Community comments: 1
Metrics
Comments: 1
HTML Views: ...
Subject Areas
Microbiology
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Group B Streptococcus (GBS) is increasingly causing invasive infections in nonpregnant adults. Previously a synergism in killing the bacteria was postulated, when a combination of penicillin plus gentamicin is used. This synergism is based on results from time-kill assays. Although, the technique of time-kill assays is known for decades, several variables may influence the stability of results, and vary for a given bacterial genus and its species. These include the incubation volume, the media, the proportion of CO2, and the use of a shaker. In this protocol, we compared these conditions with each other and outline the test results of the corresponding time-kill assays.
sowmya TN
commented on 26 April, 2020
I need a clarification regarding result reading. when we have converted the cfu/ml into log values , do we have to find the difference of the log cfu/ml of the antibiotics with the log values of the starting inoculum? the answer would be appreciated and valued. thank you