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Method Article
Parham Sendi
Institute for Infectious Diseases, University of Bern, Switzerland
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Group B Streptococcus (GBS) is increasingly causing invasive infections in nonpregnant adults. Previously a synergism in killing the bacteria was postulated, when a combination of penicillin plus gentamicin is used. This synergism is based on results from time-kill assays. Although, the technique of time-kill assays is known for decades, several variables may influence the stability of results, and vary for a given bacterial genus and its species. These include the incubation volume, the media, the proportion of CO2, and the use of a shaker. In this protocol, we compared these conditions with each other and outline the test results of the corresponding time-kill assays.
Keywords
Time kill assays, Synergism, Group B Streptococcus
The authors declare no competing financial interest.
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Version History
CURRENT STATUS: Posted
Version 1
Posted 28 Dec, 2015
Community comments: 1
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Subject Areas
Microbiology
Method Article
Parham Sendi
Institute for Infectious Diseases, University of Bern, Switzerland
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 28 Dec, 2015
Community comments: 1
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Microbiology
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Group B Streptococcus (GBS) is increasingly causing invasive infections in nonpregnant adults. Previously a synergism in killing the bacteria was postulated, when a combination of penicillin plus gentamicin is used. This synergism is based on results from time-kill assays. Although, the technique of time-kill assays is known for decades, several variables may influence the stability of results, and vary for a given bacterial genus and its species. These include the incubation volume, the media, the proportion of CO2, and the use of a shaker. In this protocol, we compared these conditions with each other and outline the test results of the corresponding time-kill assays.
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