Fabrication of a Corneal Epithelial Cell Sheet from Human Pluripotent Stem Cells by a Method Based on Spontaneous Ocular Cell Differentiation

doi:10.1038/protex.2016.009

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Method Article

Ryuhei Hayashi, Kohji Nishida, Yuki Ishikawa, Ryosuke Katori, Yuki Taniwaki, Motokazu Tsujikawa, Andrew J. Quantock

Ryuhei Hayashi

Department of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine

Corresponding Author

Kohji Nishida

Department of Ophthalmology, Osaka University Graduate School of Medicine

Corresponding Author

Yuki Ishikawa

Department of Ophthalmology, Osaka University Graduate School of Medicine

Ryosuke Katori

Department of Ophthalmology, Osaka University Graduate School of Medicine

Yuki Taniwaki

Department of Ophthalmology, Osaka University Graduate School of Medicine

Motokazu Tsujikawa

Department of Ophthalmology, Osaka University Graduate School of Medicine

Andrew J. Quantock

School of Optometry and Vision Sciences, College of Biomedical and Life Sciences, Cardiff University

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We have established a novel culture system to induce the formation of corneal epithelium through the spontaneous development of ocular cells from human induced pluripotent stem cells (hiPSCs). The hiPSC derived-corneal epithelial cells (hiCECs) can be purified by FACS with specific antibodies and have the ability to reconstruct functional corneal epithelium expressing p63, K12, and PAX6.

Keywords

iPS cells, ocular surface, corneal epithelium

The authors declare no competing financial interests.

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Method Article

Ryuhei Hayashi, Kohji Nishida, Yuki Ishikawa, Ryosuke Katori, Yuki Taniwaki, Motokazu Tsujikawa, Andrew J. Quantock

Ryuhei Hayashi

Department of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine

Corresponding Author

Kohji Nishida

Department of Ophthalmology, Osaka University Graduate School of Medicine

Corresponding Author

Yuki Ishikawa

Department of Ophthalmology, Osaka University Graduate School of Medicine

Ryosuke Katori

Department of Ophthalmology, Osaka University Graduate School of Medicine

Yuki Taniwaki

Department of Ophthalmology, Osaka University Graduate School of Medicine

Motokazu Tsujikawa

Department of Ophthalmology, Osaka University Graduate School of Medicine

Andrew J. Quantock

School of Optometry and Vision Sciences, College of Biomedical and Life Sciences, Cardiff University

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An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Ryuhei Hayashi, Kohji Nishida, Yuki Ishikawa, Ryosuke Katori, Yuki Taniwaki, Motokazu Tsujikawa, Andrew J. Quantock

Ryuhei Hayashi

Department of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine

Corresponding Author

Kohji Nishida

Department of Ophthalmology, Osaka University Graduate School of Medicine

Corresponding Author

Yuki Ishikawa

Department of Ophthalmology, Osaka University Graduate School of Medicine

Ryosuke Katori

Department of Ophthalmology, Osaka University Graduate School of Medicine

Yuki Taniwaki

Department of Ophthalmology, Osaka University Graduate School of Medicine

Motokazu Tsujikawa

Department of Ophthalmology, Osaka University Graduate School of Medicine

Andrew J. Quantock

School of Optometry and Vision Sciences, College of Biomedical and Life Sciences, Cardiff University

DOI:

10.1038/protex.2016.009

Download PDF

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Abstract

Keywords

iPS cells, ocular surface, corneal epithelium

Introduction

Reagents

Equipment

Procedure

Timing

Troubleshooting

Anticipated Results

References

Acknowledgements

Competing Interests

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An open repository of community-contributed protocols sponsored by Nature Research.

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Method Article

Ryuhei Hayashi, Kohji Nishida, Yuki Ishikawa, Ryosuke Katori, Yuki Taniwaki, Motokazu Tsujikawa, Andrew J. Quantock

Ryuhei Hayashi

Department of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine

Corresponding Author

Kohji Nishida

Department of Ophthalmology, Osaka University Graduate School of Medicine

Corresponding Author

Yuki Ishikawa

Department of Ophthalmology, Osaka University Graduate School of Medicine

Ryosuke Katori

Department of Ophthalmology, Osaka University Graduate School of Medicine

Yuki Taniwaki

Department of Ophthalmology, Osaka University Graduate School of Medicine

Motokazu Tsujikawa

Department of Ophthalmology, Osaka University Graduate School of Medicine

Andrew J. Quantock

School of Optometry and Vision Sciences, College of Biomedical and Life Sciences, Cardiff University

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