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Method Article
Fabrication of a Corneal Epithelial Cell Sheet from Human Pluripotent Stem Cells by a Method Based on Spontaneous Ocular Cell Differentiation
Ryuhei Hayashi
Department of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine
Corresponding Author
Kohji Nishida
Department of Ophthalmology, Osaka University Graduate School of Medicine
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
We have established a novel culture system to induce the formation of corneal epithelium through the spontaneous development of ocular cells from human induced pluripotent stem cells (hiPSCs). The hiPSC derived-corneal epithelial cells (hiCECs) can be purified by FACS with specific antibodies and have the ability to reconstruct functional corneal epithelium expressing p63, K12, and PAX6.
Keywords
iPS cells, ocular surface, corneal epithelium
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This is a preprint. It has not completed peer review.
Method Article
Fabrication of a Corneal Epithelial Cell Sheet from Human Pluripotent Stem Cells by a Method Based on Spontaneous Ocular Cell Differentiation
Ryuhei Hayashi
Department of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine
Corresponding Author
Kohji Nishida
Department of Ophthalmology, Osaka University Graduate School of Medicine
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 18 Feb, 2016
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
We have established a novel culture system to induce the formation of corneal epithelium through the spontaneous development of ocular cells from human induced pluripotent stem cells (hiPSCs). The hiPSC derived-corneal epithelial cells (hiCECs) can be purified by FACS with specific antibodies and have the ability to reconstruct functional corneal epithelium expressing p63, K12, and PAX6.