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Method Article
Alejandro Soto-Gutierrez
University of Pittsburgh
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Auxiliary liver transplantation is an effective solution currently available for acute liver failure and liver-based metabolic diseases. It ensures sufficient functioning liver mass to allow for native liver recovery, preserves native liver function in case of liver graft loss and the remaining donor liver can be transplanted into a second. Therefore, an animal model of this surgery offers unique and exciting opportunities to delineate and evaluate the underlying mechanisms and therapeutic interventions that can contribute to graft regeneration. Here we describe a standardized procedure for rat model of donor liver graft surgery (40–50 min operative time) and auxiliary partial liver transplantation (100–120 min operative time), which, to a high degree, resembles operations in humans. We also provide detailed protocols for both pre- and postoperative techniques that ensure a high success rate in the operations. This protocol provides the opportunity to mechanistically investigate liver regeneration in auxiliary transplantation.
Keywords
Auxiliary Liver Transplantation
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Table 1. Troubleshooting table Troubleshooting table Troubleshooting table
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Supplementary Figure 1 Schematic drawing of the rat liver vascular anatomy and retrorsine-conditioning protocol. Abbreviations: CeA, celiac artery; SMV, superior mesenteric vein; SMA, superior mesenteric artery; LGA, left gastric artery; LGV, left gastric vein; SpA, splenic artery; SpV, splenic vein; lt IPV, left inferior phrenic vein.
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Version History
CURRENT STATUS: Posted
Version 1
Posted 22 Dec, 2015
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Subject Areas
Biological techniques
Method Article
Alejandro Soto-Gutierrez
University of Pittsburgh
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 22 Dec, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Auxiliary liver transplantation is an effective solution currently available for acute liver failure and liver-based metabolic diseases. It ensures sufficient functioning liver mass to allow for native liver recovery, preserves native liver function in case of liver graft loss and the remaining donor liver can be transplanted into a second. Therefore, an animal model of this surgery offers unique and exciting opportunities to delineate and evaluate the underlying mechanisms and therapeutic interventions that can contribute to graft regeneration. Here we describe a standardized procedure for rat model of donor liver graft surgery (40–50 min operative time) and auxiliary partial liver transplantation (100–120 min operative time), which, to a high degree, resembles operations in humans. We also provide detailed protocols for both pre- and postoperative techniques that ensure a high success rate in the operations. This protocol provides the opportunity to mechanistically investigate liver regeneration in auxiliary transplantation.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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