This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
Surgical Model of Auxiliary Partial Liver Transplantation in the Rat
Alejandro Soto-Gutierrez
University of Pittsburgh
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Auxiliary liver transplantation is an effective solution currently available for acute liver failure and liver-based metabolic diseases. It ensures sufficient functioning liver mass to allow for native liver recovery, preserves native liver function in case of liver graft loss and the remaining donor liver can be transplanted into a second. Therefore, an animal model of this surgery offers unique and exciting opportunities to delineate and evaluate the underlying mechanisms and therapeutic interventions that can contribute to graft regeneration. Here we describe a standardized procedure for rat model of donor liver graft surgery (40–50 min operative time) and auxiliary partial liver transplantation (100–120 min operative time), which, to a high degree, resembles operations in humans. We also provide detailed protocols for both pre- and postoperative techniques that ensure a high success rate in the operations. This protocol provides the opportunity to mechanistically investigate liver regeneration in auxiliary transplantation.
Keywords
Auxiliary Liver Transplantation
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.docx
Table 1. Troubleshooting table Troubleshooting table Troubleshooting table
- supplement0.png
Supplementary Figure 1 Schematic drawing of the rat liver vascular anatomy and retrorsine-conditioning protocol. Abbreviations: CeA, celiac artery; SMV, superior mesenteric vein; SMA, superior mesenteric artery; LGA, left gastric artery; LGV, left gastric vein; SpA, splenic artery; SpV, splenic vein; lt IPV, left inferior phrenic vein.
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 22 Dec, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
Learn more about our company.
This is a preprint. It has not completed peer review.
Method Article
Surgical Model of Auxiliary Partial Liver Transplantation in the Rat
Alejandro Soto-Gutierrez
University of Pittsburgh
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 22 Dec, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Auxiliary liver transplantation is an effective solution currently available for acute liver failure and liver-based metabolic diseases. It ensures sufficient functioning liver mass to allow for native liver recovery, preserves native liver function in case of liver graft loss and the remaining donor liver can be transplanted into a second. Therefore, an animal model of this surgery offers unique and exciting opportunities to delineate and evaluate the underlying mechanisms and therapeutic interventions that can contribute to graft regeneration. Here we describe a standardized procedure for rat model of donor liver graft surgery (40–50 min operative time) and auxiliary partial liver transplantation (100–120 min operative time), which, to a high degree, resembles operations in humans. We also provide detailed protocols for both pre- and postoperative techniques that ensure a high success rate in the operations. This protocol provides the opportunity to mechanistically investigate liver regeneration in auxiliary transplantation.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5