In this communication, we propose a design with the use of FIAsH-EDT2 along with cysteine tagged biomolecules as a nanoswitch by coupling with multi-functional nanoparticle. Use of such nanoswitches in combination with nanoparticles that could be a quantum dot or metallic or with magnetic properties allows multiplexed applications. By using cysteine-tag, the target biomolecules can be broad and generic. In order to generate such a nanoswitch-biomolecule complex, we propose additional linker attachment to the FIAsH-EDT2 such as maleimide (for thiol interaction), NH2 (for COOH interaction) using bioconjugate chemistry for further nanoparticle interaction. Here we also propose the coupling of streptavidin or biotin with FIAsH-EDT2 for surface functionalized platform as a biosensor or as a diagnostic application for systems biology. Using the established concept of FIAsH-EDT2 based binding with cysteine tagged biomolecules that result in fluorescence ‘ON or OFF’ system, we aim to establish a generic nanoswitch that can be used for multiple applications. Some of the key applications include multiplex fluorescence bio-sensing along with preclinical applications like live- cell imaging and in-vivo analysis. Additionally, by using the physical properties of the nanoparticle, the nanoswitch can also be applied as a generic analyte towards diagnostic applications for systems biology with a broad substrate range.
At the author's request, Niamat Ali Khan has been removed from the author list.
- Bronwen Dekker, Senior Editor, Nature Protocols, 15 March 2015