RNA isolation using the miRNeasy Micro Kit

doi:10.1038/protex.2015.115

This is a protocol preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.

Method Article

Louise C. Laurent, Roger P. Alexander

Louise C. Laurent

University of California San Diego, Sanford Consortium for Regenerative Medicine, 2880 Torrey Pines Scenic Drive, La Jolla, CA

Corresponding Author

Roger P. Alexander

Sample and Assay Standards Working Group of the Extracellular RNA Communication Consortium

DOI:

10.1038/protex.2015.115

Download PDF

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

This protocol describes how to isolate RNA from biofluids using the miRNeasy Micro Kit in order to detect, identify and quantify extracellular RNA.

Keywords

exRNA, miRNeasy

The authors declare no competing financial interests.

Version History

CURRENT STATUS: Posted

Version 1

Posted 21 Dec, 2015

No community comments so far

Metrics

Comments: 0

HTML Views: ...

Subject Areas

Cell biology

Biological techniques

Molecular Biology

Associated Publications

Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium
by Louise C. Laurent, Asim B. Abdel-Mageed, P. David Adelson, +12

Method Article

Louise C. Laurent, Roger P. Alexander

Louise C. Laurent

University of California San Diego, Sanford Consortium for Regenerative Medicine, 2880 Torrey Pines Scenic Drive, La Jolla, CA

Corresponding Author

Roger P. Alexander

Sample and Assay Standards Working Group of the Extracellular RNA Communication Consortium

Version History

CURRENT STATUS: Posted

Version 1

Posted 21 Dec, 2015

No community comments so far

MetricsComments: 0HTML Views: ...

Subject Areas

Cell biology

Biological techniques

Molecular Biology

DOI:

10.1038/protex.2015.115

Download PDF

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

This protocol describes how to isolate RNA from biofluids using the miRNeasy Micro Kit in order to detect, identify and quantify extracellular RNA.

Associated Publications

Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium
by Louise C. Laurent, Asim B. Abdel-Mageed, P. David Adelson, +12

Method Article

Louise C. Laurent, Roger P. Alexander

Louise C. Laurent

University of California San Diego, Sanford Consortium for Regenerative Medicine, 2880 Torrey Pines Scenic Drive, La Jolla, CA

Corresponding Author

Roger P. Alexander

Sample and Assay Standards Working Group of the Extracellular RNA Communication Consortium

DOI:

10.1038/protex.2015.115

Download PDF

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Abstract

Keywords

exRNA, miRNeasy

Introduction

Reagents

Equipment

Procedure

Troubleshooting

Acknowledgements

Competing Interests

Comments

Version History

CURRENT STATUS: Posted

Version 1

No community comments so far

Metrics

Comments: 0

HTML Views: ...

Subject Areas

Cell biology

Biological techniques

Molecular Biology

Associated Publications

Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium

by Louise C. Laurent, Asim B. Abdel-Mageed, P. David Adelson, +12

Method Article

Louise C. Laurent, Roger P. Alexander

Louise C. Laurent

University of California San Diego, Sanford Consortium for Regenerative Medicine, 2880 Torrey Pines Scenic Drive, La Jolla, CA

Corresponding Author

Roger P. Alexander

Sample and Assay Standards Working Group of the Extracellular RNA Communication Consortium

Badges

Peer Review Timeline

Version History

CURRENT STATUS: Posted

Version 1

No community comments so far

Metrics

Metrics

Comments: 0

HTML Views: ...

Subject Areas

Subject Areas

Cell biology

Biological techniques

Molecular Biology

DOI & PDF

DOI:

10.1038/protex.2015.115

Download PDF

License

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Declarations

Abstract

Introduction

Reagents

Equipment

Procedure

Troubleshooting

Acknowledgements

Comments

Related Articles

Associated Publications

Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium

by Louise C. Laurent, Asim B. Abdel-Mageed, P. David Adelson, +12

Privacy Policy