Cell Culture Supernatant Collection

doi:10.1038/protex.2015.107

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Method Article

Louise C. Laurent, Roger P. Alexander

Louise C. Laurent

University of California San Diego, Sanford Consortium for Regenerative Medicine, 2880 Torrey Pines Scenic Drive, La Jolla, CA

Corresponding Author

Roger P. Alexander

Sample and Assay Standards Working Group of the Extracellular RNA Communication Consortium

Corresponding Author

DOI:

10.1038/protex.2015.107

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This protocol describes how to collect supernatant from a cell culture preparation in order to detect, identify and quantify extracellular RNA from the sample.

Keywords

exRNA, sample collection

The authors declare no competing financial interests.

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Cell biology

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Associated Publications

Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium
by Louise C. Laurent, Asim B. Abdel-Mageed, P. David Adelson, +12

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Louise C. Laurent, Roger P. Alexander

Louise C. Laurent

University of California San Diego, Sanford Consortium for Regenerative Medicine, 2880 Torrey Pines Scenic Drive, La Jolla, CA

Corresponding Author

Roger P. Alexander

Sample and Assay Standards Working Group of the Extracellular RNA Communication Consortium

Corresponding Author

Version History

CURRENT STATUS: Posted

Version 1

Posted 21 Dec, 2015

No community comments so far

MetricsComments: 0HTML Views: ...

Subject Areas

Cell biology

Biological techniques

DOI:

10.1038/protex.2015.107

Download PDF

The full text of this article is available to read as a PDF.

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

This protocol describes how to collect supernatant from a cell culture preparation in order to detect, identify and quantify extracellular RNA from the sample.

The authors declare no competing financial interests.

Associated Publications

Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium
by Louise C. Laurent, Asim B. Abdel-Mageed, P. David Adelson, +12

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Louise C. Laurent, Roger P. Alexander

Louise C. Laurent

University of California San Diego, Sanford Consortium for Regenerative Medicine, 2880 Torrey Pines Scenic Drive, La Jolla, CA

Corresponding Author

Roger P. Alexander

Sample and Assay Standards Working Group of the Extracellular RNA Communication Consortium

Corresponding Author

DOI:

10.1038/protex.2015.107

Download PDF

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Abstract

Keywords

exRNA, sample collection

Introduction

Procedure

Competing Interests

Comments

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CURRENT STATUS: Posted

Version 1

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Subject Areas

Cell biology

Biological techniques

Associated Publications

Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium

by Louise C. Laurent, Asim B. Abdel-Mageed, P. David Adelson, +12

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Louise C. Laurent, Roger P. Alexander

Louise C. Laurent

University of California San Diego, Sanford Consortium for Regenerative Medicine, 2880 Torrey Pines Scenic Drive, La Jolla, CA

Corresponding Author

Roger P. Alexander

Sample and Assay Standards Working Group of the Extracellular RNA Communication Consortium

Corresponding Author

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CURRENT STATUS: Posted

Version 1

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Subject Areas

Subject Areas

Cell biology

Biological techniques

DOI & PDF

DOI:

10.1038/protex.2015.107

Download PDF

License

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Declarations

Abstract

Introduction

Procedure

Competing Interests

Comments

Related Articles

Associated Publications

Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium

by Louise C. Laurent, Asim B. Abdel-Mageed, P. David Adelson, +12

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