This is a protocol preprint. Preprints are preliminary reports that have not undergone peer review. They should not be considered conclusive, used to inform clinical practice, or referenced by the media as validated information.
Method Article
The Lemna Toxicity Test: Guideline of a New Test System Based on Root Re-Growth
Taejun Han
Taejun Han, Incheon National University
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Declarations:
A new phytotoxicity test method based on Lemna root elongation has been developed. Tests with aquatic plants have, typically, favored measurements on fronds (e.g. frond number, area, biomass) rather than on roots, due, in part, to issues associated with handling fragile roots and the time-consuming procedures of selecting roots with identical root lengths. The new test method differs from the traditional procedures by removing all roots from fronds prior to exposure to toxicants with subsequent measurements on newly developed roots. The root re-growth bioassay also differs from three internationally standardized methods (ISO, OCED and US EPA) in that it is completed within a shorter time period (72 h), the required volume of test solutions is only 3.0 mL and non-axenic plants are used. Our results show that the Lemna root method is a simple, rapid, cost-effective, sensitive and precise bioassay to assess the toxic risks of environmental contaminants.
Keywords
Aquatic plants, Lemna spp., Root, Toxicity
Figure 1
Figure 2
Figure 3
Figure 4
Version History
CURRENT STATUS: Posted
Version 1
Posted 12 Oct, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Associated Publications
A novel bioassay using root re-growth in Lemna
by Areum Park, Youn-Jung Kim, Eun-Mi Choi, +2
Method Article
The Lemna Toxicity Test: Guideline of a New Test System Based on Root Re-Growth
Taejun Han
Taejun Han, Incheon National University
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 12 Oct, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Declarations:
A new phytotoxicity test method based on Lemna root elongation has been developed. Tests with aquatic plants have, typically, favored measurements on fronds (e.g. frond number, area, biomass) rather than on roots, due, in part, to issues associated with handling fragile roots and the time-consuming procedures of selecting roots with identical root lengths. The new test method differs from the traditional procedures by removing all roots from fronds prior to exposure to toxicants with subsequent measurements on newly developed roots. The root re-growth bioassay also differs from three internationally standardized methods (ISO, OCED and US EPA) in that it is completed within a shorter time period (72 h), the required volume of test solutions is only 3.0 mL and non-axenic plants are used. Our results show that the Lemna root method is a simple, rapid, cost-effective, sensitive and precise bioassay to assess the toxic risks of environmental contaminants.
Figure 1
Figure 2
Figure 3
Figure 4
Associated Publications
A novel bioassay using root re-growth in Lemna
by Areum Park, Youn-Jung Kim, Eun-Mi Choi, +2