Evaluation of Molecular Tension Sensors using Single-Molecule Force Spectroscopy and Live Cell FRET imaging
This protocol describes the calibration of fluorescence resonance energy transfer (FRET)-based tension sensors using single-molecule force spectroscopy. We also outline the evaluation of molecular tension sensors in living cells by fluorescence lifetime microscopy (FLIM) and ratiometric FRET imaging.
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Posted 03 Nov, 2015
Evaluation of Molecular Tension Sensors using Single-Molecule Force Spectroscopy and Live Cell FRET imaging
Posted 03 Nov, 2015
This protocol describes the calibration of fluorescence resonance energy transfer (FRET)-based tension sensors using single-molecule force spectroscopy. We also outline the evaluation of molecular tension sensors in living cells by fluorescence lifetime microscopy (FLIM) and ratiometric FRET imaging.
Figure 1
Figure 2
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