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Method Article
Evaluation of Molecular Tension Sensors using Single-Molecule Force Spectroscopy and Live Cell FRET imaging
Matthias Rief
Technical University of Munich
Corresponding Author
Carsten Grashoff
Carsten Grashoff Lab
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes the calibration of fluorescence resonance energy transfer (FRET)-based tension sensors using single-molecule force spectroscopy. We also outline the evaluation of molecular tension sensors in living cells by fluorescence lifetime microscopy (FLIM) and ratiometric FRET imaging.
Keywords
FRET-based tension sensor, Single-molecule force spectroscopy
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Cell biology
Associated Publications
Extracellular rigidity sensing by talin isoform-specific mechanical linkages
by Katharina Austen, Pia Ringer, Alexander Mehlich, +7
Nature Cell Biology (03 November, 2015)
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This is a preprint. It has not completed peer review.
Method Article
Evaluation of Molecular Tension Sensors using Single-Molecule Force Spectroscopy and Live Cell FRET imaging
Matthias Rief
Technical University of Munich
Corresponding Author
Carsten Grashoff
Carsten Grashoff Lab
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 03 Nov, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Cell biology
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes the calibration of fluorescence resonance energy transfer (FRET)-based tension sensors using single-molecule force spectroscopy. We also outline the evaluation of molecular tension sensors in living cells by fluorescence lifetime microscopy (FLIM) and ratiometric FRET imaging.
Figure 1
Figure 2
Associated Publications
Extracellular rigidity sensing by talin isoform-specific mechanical linkages
by Katharina Austen, Pia Ringer, Alexander Mehlich, +7
Nature Cell Biology (03 November, 2015)