A protocol for the role of TNF-α on regulatory T cell function
In vitro Treg suppression assays are performed to determine the functional effect of TNF-α on Treg cells. In addition to the co-culture condition to measure the effect of TNF-α on human Treg by mixing them with Teff cells, parallel experiments were conducted where TNF-α was washed away prior to co-culture (pre-treatment condition).
Posted 12 Oct, 2015
A protocol for the role of TNF-α on regulatory T cell function
Posted 12 Oct, 2015
In vitro Treg suppression assays are performed to determine the functional effect of TNF-α on Treg cells. In addition to the co-culture condition to measure the effect of TNF-α on human Treg by mixing them with Teff cells, parallel experiments were conducted where TNF-α was washed away prior to co-culture (pre-treatment condition).
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