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Method Article
A protocol for the role of TNF-α on regulatory T cell function
Jingwu Zhang
BioSciKin, Nanjing, China.
Corresponding Author
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This work is licensed under a CC BY-NC 3.0 License. Read Full License
In vitro Treg suppression assays are performed to determine the functional effect of TNF-α on Treg cells. In addition to the co-culture condition to measure the effect of TNF-α on human Treg by mixing them with Teff cells, parallel experiments were conducted where TNF-α was washed away prior to co-culture (pre-treatment condition).
Keywords
TNF-α, Treg cells, Teff cells
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This is a preprint. It has not completed peer review.
Method Article
A protocol for the role of TNF-α on regulatory T cell function
Jingwu Zhang
BioSciKin, Nanjing, China.
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 12 Oct, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
In vitro Treg suppression assays are performed to determine the functional effect of TNF-α on Treg cells. In addition to the co-culture condition to measure the effect of TNF-α on human Treg by mixing them with Teff cells, parallel experiments were conducted where TNF-α was washed away prior to co-culture (pre-treatment condition).