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Method Article
Jingwu Zhang
BioSciKin, Nanjing, China.
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
In vitro Treg suppression assays are performed to determine the functional effect of TNF-α on Treg cells. In addition to the co-culture condition to measure the effect of TNF-α on human Treg by mixing them with Teff cells, parallel experiments were conducted where TNF-α was washed away prior to co-culture (pre-treatment condition).
Keywords
TNF-α, Treg cells, Teff cells
The authors declare no competing financial interests.
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Subject Areas
Biological techniques
Method Article
Jingwu Zhang
BioSciKin, Nanjing, China.
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 12 Oct, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
In vitro Treg suppression assays are performed to determine the functional effect of TNF-α on Treg cells. In addition to the co-culture condition to measure the effect of TNF-α on human Treg by mixing them with Teff cells, parallel experiments were conducted where TNF-α was washed away prior to co-culture (pre-treatment condition).
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