This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
Reprogramming of mouse fibroblasts into iPSCs
Sheng Ding
Gladstone Institute of Cardiovascular Disease, San Francisco, California, 94158, USA
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol is derived from the original method to generate induced pluripotent stem cells (iPSCs)1, 2, and describes the reprogramming of mouse embryonic fibroblasts (MEFs) and mouse tail tip fibroblasts (TTFs), using pMXs-based retrovirus encoding Oct4, Sox2, Klf4, and c-Myc.
Keywords
Reprogramming, induced pluripotent stem cell, fibroblast.
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 14 Sep, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Cell biology
Associated Publications
Atg5-independent autophagy regulates mitochondrial clearance and is essential for iPSC reprogramming
by Tianhua Ma, Jun Li, Yue Xu, +13
Nature Cell Biology (23 November, 2015)
Learn more about our company.
This is a preprint. It has not completed peer review.
Method Article
Reprogramming of mouse fibroblasts into iPSCs
Sheng Ding
Gladstone Institute of Cardiovascular Disease, San Francisco, California, 94158, USA
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 14 Sep, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Cell biology
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol is derived from the original method to generate induced pluripotent stem cells (iPSCs)1, 2, and describes the reprogramming of mouse embryonic fibroblasts (MEFs) and mouse tail tip fibroblasts (TTFs), using pMXs-based retrovirus encoding Oct4, Sox2, Klf4, and c-Myc.
Associated Publications
Atg5-independent autophagy regulates mitochondrial clearance and is essential for iPSC reprogramming
by Tianhua Ma, Jun Li, Yue Xu, +13
Nature Cell Biology (23 November, 2015)