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Method Article
Sheng Ding
Gladstone Institute of Cardiovascular Disease, San Francisco, California, 94158, USA
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol is derived from the original method to generate induced pluripotent stem cells (iPSCs)1, 2, and describes the reprogramming of mouse embryonic fibroblasts (MEFs) and mouse tail tip fibroblasts (TTFs), using pMXs-based retrovirus encoding Oct4, Sox2, Klf4, and c-Myc.
Keywords
Reprogramming, induced pluripotent stem cell, fibroblast.
The authors declare no conflicting financial interests.
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Posted 14 Sep, 2015
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Cell biology
Associated Publications
Atg5-independent autophagy regulates mitochondrial clearance and is essential for iPSC reprogramming
by Tianhua Ma, Jun Li, Yue Xu, +13
Nature Cell Biology (23 November, 2015)
Method Article
Sheng Ding
Gladstone Institute of Cardiovascular Disease, San Francisco, California, 94158, USA
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 14 Sep, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Cell biology
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol is derived from the original method to generate induced pluripotent stem cells (iPSCs)1, 2, and describes the reprogramming of mouse embryonic fibroblasts (MEFs) and mouse tail tip fibroblasts (TTFs), using pMXs-based retrovirus encoding Oct4, Sox2, Klf4, and c-Myc.
Loading...
Associated Publications
Atg5-independent autophagy regulates mitochondrial clearance and is essential for iPSC reprogramming
by Tianhua Ma, Jun Li, Yue Xu, +13
Nature Cell Biology (23 November, 2015)