Reprogramming of mouse fibroblasts into iPSCs

doi:10.1038/protex.2015.086

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Method Article

Sheng Ding, Tianhua Ma, Jun Li

Sheng Ding

Gladstone Institute of Cardiovascular Disease, San Francisco, California, 94158, USA

Corresponding Author

Tianhua Ma

Gladstone Institute of Cardiovascular Disease, San Francisco, California, 94158, USA

Jun Li

Institute of Immunology, PLA, Third Military Medical University, Chongqing, China, 400038

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This protocol is derived from the original method to generate induced pluripotent stem cells (iPSCs)^{1, 2}, and describes the reprogramming of mouse embryonic fibroblasts (MEFs) and mouse tail tip fibroblasts (TTFs), using pMXs-based retrovirus encoding Oct4, Sox2, Klf4, and c-Myc.

Keywords

Reprogramming, induced pluripotent stem cell, fibroblast.

The authors declare no conflicting financial interests.

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Associated Publications

Atg5-independent autophagy regulates mitochondrial clearance and is essential for iPSC reprogramming
by Tianhua Ma, Jun Li, Yue Xu, +13
Nature Cell Biology (23 November, 2015)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Sheng Ding, Tianhua Ma, Jun Li

Sheng Ding

Gladstone Institute of Cardiovascular Disease, San Francisco, California, 94158, USA

Corresponding Author

Tianhua Ma

Gladstone Institute of Cardiovascular Disease, San Francisco, California, 94158, USA

Jun Li

Institute of Immunology, PLA, Third Military Medical University, Chongqing, China, 400038

Version History

CURRENT STATUS: Posted

Version 1

Posted 14 Sep, 2015

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Cell biology

DOI:

10.1038/protex.2015.086

Download PDF

The full text of this article is available to read as a PDF.

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

The authors declare no conflicting financial interests.

Associated Publications

Atg5-independent autophagy regulates mitochondrial clearance and is essential for iPSC reprogramming
by Tianhua Ma, Jun Li, Yue Xu, +13
Nature Cell Biology (23 November, 2015)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Sheng Ding, Tianhua Ma, Jun Li

Sheng Ding

Gladstone Institute of Cardiovascular Disease, San Francisco, California, 94158, USA

Corresponding Author

Tianhua Ma

Gladstone Institute of Cardiovascular Disease, San Francisco, California, 94158, USA

Jun Li

Institute of Immunology, PLA, Third Military Medical University, Chongqing, China, 400038

DOI:

10.1038/protex.2015.086

Download PDF

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Abstract

Keywords

Reprogramming, induced pluripotent stem cell, fibroblast.

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Acknowledgements

Competing Interests

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Associated Publications

Atg5-independent autophagy regulates mitochondrial clearance and is essential for iPSC reprogramming

by Tianhua Ma, Jun Li, Yue Xu, +13

Nature Cell Biology (23 November, 2015)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Sheng Ding, Tianhua Ma, Jun Li

Sheng Ding

Gladstone Institute of Cardiovascular Disease, San Francisco, California, 94158, USA

Corresponding Author

Tianhua Ma

Gladstone Institute of Cardiovascular Disease, San Francisco, California, 94158, USA

Jun Li

Institute of Immunology, PLA, Third Military Medical University, Chongqing, China, 400038

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DOI:

10.1038/protex.2015.086

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License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

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Abstract

Reagents

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References

Acknowledgements

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Associated Publications

Atg5-independent autophagy regulates mitochondrial clearance and is essential for iPSC reprogramming