Reprogramming of mouse fibroblasts into iPSCs
This protocol is derived from the original method to generate induced pluripotent stem cells (iPSCs)1, 2, and describes the reprogramming of mouse embryonic fibroblasts (MEFs) and mouse tail tip fibroblasts (TTFs), using pMXs-based retrovirus encoding Oct4, Sox2, Klf4, and c-Myc.
Posted 14 Sep, 2015
Reprogramming of mouse fibroblasts into iPSCs
Posted 14 Sep, 2015
This protocol is derived from the original method to generate induced pluripotent stem cells (iPSCs)1, 2, and describes the reprogramming of mouse embryonic fibroblasts (MEFs) and mouse tail tip fibroblasts (TTFs), using pMXs-based retrovirus encoding Oct4, Sox2, Klf4, and c-Myc.
© Research Square 2021