This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
Counting marine microbes with Guava Easy-Cyte 96 well plate reading flow cytometer
H. James Tripp
Oregon State University
Corresponding Author
Figure 1
Ssemakalu Cornelius
commented on 07 June, 2009
Wonderful and straight forward however is the SYBR green the one supplied by invitrogen? In which case is the working concentration 5x? Thank you
Kevin Vergin
commented on 24 September, 2009
true, we use Sybr Green from Invitrogen. We use a 1:20 or 1:30 dilution of the stock and stain cells at a 1:100 dilution so it is a final dilution of 1:2000 or 3000. It is possible that the 1:3000 dilution gives less background noise. We stain for at least one hour before counting.
Ssemakalu Cornelius
commented on 07 June, 2009
Wonderful and straight forward however is the SYBR green the one supplied by invitrogen? In which case is the working concentration 5x? Thank you
Kevin Vergin
commented on 24 September, 2009
true, we use Sybr Green from Invitrogen. We use a 1:20 or 1:30 dilution of the stock and stain cells at a 1:100 dilution so it is a final dilution of 1:2000 or 3000. It is possible that the 1:3000 dilution gives less background noise. We stain for at least one hour before counting.
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 18 Mar, 2008
Community comments: 4
Metrics
Comments: 4
HTML Views: ...
Subject Areas
Microbiology
Associated Publications
SAR11 marine bacteria require exogenous reduced sulphur for growth
by H. James Tripp, Joshua B. Kitner, Michael S. Schwalbach, +2
Nature (31 January, 2008)
Learn more about our company.
This is a preprint. It has not completed peer review.
Method Article
Counting marine microbes with Guava Easy-Cyte 96 well plate reading flow cytometer
H. James Tripp
Oregon State University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 18 Mar, 2008
Community comments: 4
Metrics
Comments: 4
HTML Views: ...
Subject Areas
Microbiology
Figure 1
Ssemakalu Cornelius
commented on 07 June, 2009
Wonderful and straight forward however is the SYBR green the one supplied by invitrogen? In which case is the working concentration 5x? Thank you
Kevin Vergin
commented on 24 September, 2009
true, we use Sybr Green from Invitrogen. We use a 1:20 or 1:30 dilution of the stock and stain cells at a 1:100 dilution so it is a final dilution of 1:2000 or 3000. It is possible that the 1:3000 dilution gives less background noise. We stain for at least one hour before counting.
Ssemakalu Cornelius
commented on 07 June, 2009
Wonderful and straight forward however is the SYBR green the one supplied by invitrogen? In which case is the working concentration 5x? Thank you
Kevin Vergin
commented on 24 September, 2009
true, we use Sybr Green from Invitrogen. We use a 1:20 or 1:30 dilution of the stock and stain cells at a 1:100 dilution so it is a final dilution of 1:2000 or 3000. It is possible that the 1:3000 dilution gives less background noise. We stain for at least one hour before counting.
Associated Publications
SAR11 marine bacteria require exogenous reduced sulphur for growth
by H. James Tripp, Joshua B. Kitner, Michael S. Schwalbach, +2
Nature (31 January, 2008)