Counting marine microbes with Guava Easy-Cyte 96 well plate reading flow cytometer
Figure 1
Wonderful and straight forward however is the SYBR green the one supplied by invitrogen? In which case is the working concentration 5x? Thank you
true, we use Sybr Green from Invitrogen. We use a 1:20 or 1:30 dilution of the stock and stain cells at a 1:100 dilution so it is a final dilution of 1:2000 or 3000. It is possible that the 1:3000 dilution gives less background noise. We stain for at least one hour before counting.
Wonderful and straight forward however is the SYBR green the one supplied by invitrogen? In which case is the working concentration 5x? Thank you
true, we use Sybr Green from Invitrogen. We use a 1:20 or 1:30 dilution of the stock and stain cells at a 1:100 dilution so it is a final dilution of 1:2000 or 3000. It is possible that the 1:3000 dilution gives less background noise. We stain for at least one hour before counting.
Posted 18 Mar, 2008
Counting marine microbes with Guava Easy-Cyte 96 well plate reading flow cytometer
Posted 18 Mar, 2008
Figure 1
Wonderful and straight forward however is the SYBR green the one supplied by invitrogen? In which case is the working concentration 5x? Thank you
true, we use Sybr Green from Invitrogen. We use a 1:20 or 1:30 dilution of the stock and stain cells at a 1:100 dilution so it is a final dilution of 1:2000 or 3000. It is possible that the 1:3000 dilution gives less background noise. We stain for at least one hour before counting.
Wonderful and straight forward however is the SYBR green the one supplied by invitrogen? In which case is the working concentration 5x? Thank you
true, we use Sybr Green from Invitrogen. We use a 1:20 or 1:30 dilution of the stock and stain cells at a 1:100 dilution so it is a final dilution of 1:2000 or 3000. It is possible that the 1:3000 dilution gives less background noise. We stain for at least one hour before counting.
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