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Method Article
Shobhit Saxena
Department of Molecular Medicine and Gene Therapy, Lund University
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Sympathoadrenergic progenitor cells (SAPs) of the peripheral nervous system (PNS) are important for normal development of the sympathetic PNS. SAPs are also thought to be the cell of origin of neuroblastoma, a sympathetic PNS-related childhood tumor. However, it remains difficult to isolate sufficient numbers of SAPs. This protocol describes methodology for the generation of SAPs by differentiating murine embryonic stem cells (ESCs). These advances in SAP generation may facilitate investigations about the normal development and malignant transformation of the sympathetic PNS.
Keywords
Neural crest stem cells, sympathoadrenergic progenitors, embryonic stem cells, CD57, GD2, flow cytometry
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The authors declare no competing financial interests.
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Associated Publications
Generation of Murine Sympathoadrenergic Progenitor-Like Cells from Embryonic Stem Cells and Postnatal Adrenal Glands
by Shobhit Saxena, Joachim Wahl, Markus S. Huber-Lang, +5
PLoS ONE (04 August, 2015)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Shobhit Saxena
Department of Molecular Medicine and Gene Therapy, Lund University
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 14 Sep, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Sympathoadrenergic progenitor cells (SAPs) of the peripheral nervous system (PNS) are important for normal development of the sympathetic PNS. SAPs are also thought to be the cell of origin of neuroblastoma, a sympathetic PNS-related childhood tumor. However, it remains difficult to isolate sufficient numbers of SAPs. This protocol describes methodology for the generation of SAPs by differentiating murine embryonic stem cells (ESCs). These advances in SAP generation may facilitate investigations about the normal development and malignant transformation of the sympathetic PNS.
Figure 1
Figure 2
Figure 3
Figure 4
The authors declare no competing financial interests.
Loading...
Associated Publications
Generation of Murine Sympathoadrenergic Progenitor-Like Cells from Embryonic Stem Cells and Postnatal Adrenal Glands
by Shobhit Saxena, Joachim Wahl, Markus S. Huber-Lang, +5
PLoS ONE (04 August, 2015)