Generation of murine sympathoadrenergic progenitor-like cells from embryonic stem cells
Sympathoadrenergic progenitor cells (SAPs) of the peripheral nervous system (PNS) are important for normal development of the sympathetic PNS. SAPs are also thought to be the cell of origin of neuroblastoma, a sympathetic PNS-related childhood tumor. However, it remains difficult to isolate sufficient numbers of SAPs. This protocol describes methodology for the generation of SAPs by differentiating murine embryonic stem cells (ESCs). These advances in SAP generation may facilitate investigations about the normal development and malignant transformation of the sympathetic PNS.
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Posted 14 Sep, 2015
Generation of murine sympathoadrenergic progenitor-like cells from embryonic stem cells
Posted 14 Sep, 2015
Sympathoadrenergic progenitor cells (SAPs) of the peripheral nervous system (PNS) are important for normal development of the sympathetic PNS. SAPs are also thought to be the cell of origin of neuroblastoma, a sympathetic PNS-related childhood tumor. However, it remains difficult to isolate sufficient numbers of SAPs. This protocol describes methodology for the generation of SAPs by differentiating murine embryonic stem cells (ESCs). These advances in SAP generation may facilitate investigations about the normal development and malignant transformation of the sympathetic PNS.
Figure 1
Figure 2
Figure 3
Figure 4
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