Analysis of patient-specific immunoglobulin proteomes and transcriptomes by PCR cloning and mass spectrometry

doi:10.1038/nprot.2008.87

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Method Article

Analysis of patient-specific immunoglobulin proteomes and transcriptomes by PCR cloning and mass spectrometry

Birgit Obermeier, Reinhard Mentele, Joachim Malotka, Klaus Dornmair

Birgit Obermeier

Institute of Clinical Neuroimmunology, Ludwig Maximilians University, D-81377 Munich, Germany; and: Department of Neuroimmunology, Max-Planck-Institute of Neurobiology, D-82152 Martinsried, Germany

Reinhard Mentele

Department for Protein Analytics, Max-Planck-Institute of Biochemistry, D-82152 Martinsried, Germany

Joachim Malotka

Department of Neuroimmunology, Max-Planck-Institute of Neurobiology, D-82152 Martinsried, Germany

Klaus Dornmair

Institute of Clinical Neuroimmunology, Ludwig Maximilians University, D-81377 Munich, Germany; and: Department of Neuroimmunology, Max-Planck-Institute of Neurobiology, D-82152 Martinsried, Germany

DOI:

10.1038/nprot.2008.87

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Associated Publications

Matching of oligoclonal immunoglobulin transcriptomes and proteomes of cerebrospinal fluid in multiple sclerosis
by Birgit Obermeier, Reinhard Mentele, Joachim Malotka, +5
Nature Medicine (22 January, 2008)

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This is a preprint. It has not completed peer review.

Method Article

Analysis of patient-specific immunoglobulin proteomes and transcriptomes by PCR cloning and mass spectrometry

Birgit Obermeier, Reinhard Mentele, Joachim Malotka, Klaus Dornmair

Birgit Obermeier

Institute of Clinical Neuroimmunology, Ludwig Maximilians University, D-81377 Munich, Germany; and: Department of Neuroimmunology, Max-Planck-Institute of Neurobiology, D-82152 Martinsried, Germany

Reinhard Mentele

Department for Protein Analytics, Max-Planck-Institute of Biochemistry, D-82152 Martinsried, Germany

Joachim Malotka

Department of Neuroimmunology, Max-Planck-Institute of Neurobiology, D-82152 Martinsried, Germany

Klaus Dornmair

Institute of Clinical Neuroimmunology, Ludwig Maximilians University, D-81377 Munich, Germany; and: Department of Neuroimmunology, Max-Planck-Institute of Neurobiology, D-82152 Martinsried, Germany

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History

CURRENT STATUS: Posted

Version 1

Posted 20 May, 2008

No community comments so far

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Biological techniques

DOI:

10.1038/nprot.2008.87

Download PDF

Associated Publications

Matching of oligoclonal immunoglobulin transcriptomes and proteomes of cerebrospinal fluid in multiple sclerosis
by Birgit Obermeier, Reinhard Mentele, Joachim Malotka, +5
Nature Medicine (22 January, 2008)

Method Article

Analysis of patient-specific immunoglobulin proteomes and transcriptomes by PCR cloning and mass spectrometry

Birgit Obermeier, Reinhard Mentele, Joachim Malotka, Klaus Dornmair

Birgit Obermeier

Institute of Clinical Neuroimmunology, Ludwig Maximilians University, D-81377 Munich, Germany; and: Department of Neuroimmunology, Max-Planck-Institute of Neurobiology, D-82152 Martinsried, Germany

Reinhard Mentele

Department for Protein Analytics, Max-Planck-Institute of Biochemistry, D-82152 Martinsried, Germany

Joachim Malotka

Department of Neuroimmunology, Max-Planck-Institute of Neurobiology, D-82152 Martinsried, Germany

Klaus Dornmair

Institute of Clinical Neuroimmunology, Ludwig Maximilians University, D-81377 Munich, Germany; and: Department of Neuroimmunology, Max-Planck-Institute of Neurobiology, D-82152 Martinsried, Germany

DOI:

10.1038/nprot.2008.87

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CURRENT STATUS: Posted

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Associated Publications

Matching of oligoclonal immunoglobulin transcriptomes and proteomes of cerebrospinal fluid in multiple sclerosis

by Birgit Obermeier, Reinhard Mentele, Joachim Malotka, +5

Nature Medicine (22 January, 2008)

Learn more about our company.

This is a preprint. It has not completed peer review.

Method Article

Analysis of patient-specific immunoglobulin proteomes and transcriptomes by PCR cloning and mass spectrometry

Birgit Obermeier, Reinhard Mentele, Joachim Malotka, Klaus Dornmair

Birgit Obermeier

Institute of Clinical Neuroimmunology, Ludwig Maximilians University, D-81377 Munich, Germany; and: Department of Neuroimmunology, Max-Planck-Institute of Neurobiology, D-82152 Martinsried, Germany

Reinhard Mentele

Department for Protein Analytics, Max-Planck-Institute of Biochemistry, D-82152 Martinsried, Germany

Joachim Malotka

Department of Neuroimmunology, Max-Planck-Institute of Neurobiology, D-82152 Martinsried, Germany

Klaus Dornmair

Institute of Clinical Neuroimmunology, Ludwig Maximilians University, D-81377 Munich, Germany; and: Department of Neuroimmunology, Max-Planck-Institute of Neurobiology, D-82152 Martinsried, Germany

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DOI:

10.1038/nprot.2008.87

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