Microfluidic device design, fabrication, and testing protocols

doi:10.1038/protex.2015.069

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Method Article

David Hoelzle, Melinda Lake, Cody Narciso, Kyle Cowdrick, Thomas Storey, Siyuan Zhang, Jeremiah Zartman

David Hoelzle

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Corresponding Author

Melinda Lake

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Cody Narciso

Department of Chemical and Biomolecular Engineering, University of Notre Dame

Kyle Cowdrick

Department of Chemical and Biomolecular Engineering, Harper Cancer Research Institute, University of Notre Dame

Thomas Storey

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Siyuan Zhang

Harper Cancer Research Institute, Department of Biological Sciences, University of Notre Dame

Jeremiah Zartman

Department of Chemical and Biomolecular Engineering, University of Notre Dame

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This protocols document describes the design considerations and software tools to design a microfluidic device, fabrication protocols for making master molds and the final polydimethylsiloxane (PDMS) device, and testing of the completed microfluidic device.

Keywords

microfluidic device, microfabrication

The authors declare no competing financial interests.

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Protocols document Microfluidic device design, fabrication, and testing protocols

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An open repository of community-contributed protocols sponsored by Nature Research.

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Method Article

David Hoelzle, Melinda Lake, Cody Narciso, Kyle Cowdrick, Thomas Storey, Siyuan Zhang, Jeremiah Zartman

David Hoelzle

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Corresponding Author

Melinda Lake

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Cody Narciso

Department of Chemical and Biomolecular Engineering, University of Notre Dame

Kyle Cowdrick

Department of Chemical and Biomolecular Engineering, Harper Cancer Research Institute, University of Notre Dame

Thomas Storey

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Siyuan Zhang

Harper Cancer Research Institute, Department of Biological Sciences, University of Notre Dame

Jeremiah Zartman

Department of Chemical and Biomolecular Engineering, University of Notre Dame

Version History

CURRENT STATUS: Posted

Version 1

Posted 07 Jul, 2015

No community comments so far

MetricsComments: 0HTML Views: ...

Subject Areas

Cell biology

Biological techniques

Microbiology

DOI:

10.1038/protex.2015.069

Download PDF

The full text of this article is available to read as a PDF.

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

The authors declare no competing financial interests.

This is a list of supplementary files associated with this preprint. Click to download.

supplement0.pdf
Protocols document Microfluidic device design, fabrication, and testing protocols

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

David Hoelzle, Melinda Lake, Cody Narciso, Kyle Cowdrick, Thomas Storey, Siyuan Zhang, Jeremiah Zartman

David Hoelzle

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Corresponding Author

Melinda Lake

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Cody Narciso

Department of Chemical and Biomolecular Engineering, University of Notre Dame

Kyle Cowdrick

Department of Chemical and Biomolecular Engineering, Harper Cancer Research Institute, University of Notre Dame

Thomas Storey

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Siyuan Zhang

Harper Cancer Research Institute, Department of Biological Sciences, University of Notre Dame

Jeremiah Zartman

Department of Chemical and Biomolecular Engineering, University of Notre Dame

DOI:

10.1038/protex.2015.069

Download PDF

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Abstract

Keywords

microfluidic device, microfabrication

Introduction

Procedure

Timing

References

Acknowledgements

Competing Interests

Supplementary Files

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Cell biology

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Microbiology

An open repository of community-contributed protocols sponsored by Nature Research.

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Method Article

David Hoelzle, Melinda Lake, Cody Narciso, Kyle Cowdrick, Thomas Storey, Siyuan Zhang, Jeremiah Zartman

David Hoelzle

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Corresponding Author

Melinda Lake

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Cody Narciso

Department of Chemical and Biomolecular Engineering, University of Notre Dame

Kyle Cowdrick

Department of Chemical and Biomolecular Engineering, Harper Cancer Research Institute, University of Notre Dame

Thomas Storey

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Siyuan Zhang

Harper Cancer Research Institute, Department of Biological Sciences, University of Notre Dame

Jeremiah Zartman

Department of Chemical and Biomolecular Engineering, University of Notre Dame

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Subject Areas

Cell biology

Biological techniques

Microbiology