Microfluidic devices are often used to perform biological assays. They have been used for functions that include to trap1, deform2 and sort3 cells. To make these tools we use fabrication processes adopted from the integrated circuit manufacturing industry to first make a master mold and using this master mold we make a complete device.4 The procedure to fabricate a master and corresponding microfluidic device is shown by the schematics in Figure 1 (See attachment "Protocols document").
The purpose of this document is to provide guidelines for first time users. This will help the reader understand what is needed to take an idea for a device through the design and fabrication process to make a complete device for testing. Section 1 presents microfluidic device design using AutoCAD. Section 2 describes the master fabrication steps (Figure 1.1-1.3). Section 3 provides the procedure to use polydimethylsiloxane (PDMS) to fabricate the finalized microfluidic device (Figure 1.4-1.5). Section 4 discusses the methods used to test the devices.