Microfluidic device design, fabrication, and testing protocols

doi:10.1038/protex.2015.069

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Method Article

Microfluidic device design, fabrication, and testing protocols

David Hoelzle, Melinda Lake, Cody Narciso, Kyle Cowdrick, Thomas Storey, Siyuan Zhang, Jeremiah Zartman

David Hoelzle

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Corresponding Author

Melinda Lake

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Cody Narciso

Department of Chemical and Biomolecular Engineering, University of Notre Dame

Kyle Cowdrick

Department of Chemical and Biomolecular Engineering, Harper Cancer Research Institute, University of Notre Dame

Thomas Storey

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Siyuan Zhang

Harper Cancer Research Institute, Department of Biological Sciences, University of Notre Dame

Jeremiah Zartman

Department of Chemical and Biomolecular Engineering, University of Notre Dame

DOI:

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This protocols document describes the design considerations and software tools to design a microfluidic device, fabrication protocols for making master molds and the final polydimethylsiloxane (PDMS) device, and testing of the completed microfluidic device.

Keywords

microfluidic device, microfabrication

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Protocols document Microfluidic device design, fabrication, and testing protocols

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This is a preprint. It has not completed peer review.

Method Article

Microfluidic device design, fabrication, and testing protocols

David Hoelzle, Melinda Lake, Cody Narciso, Kyle Cowdrick, Thomas Storey, Siyuan Zhang, Jeremiah Zartman

David Hoelzle

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Corresponding Author

Melinda Lake

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Cody Narciso

Department of Chemical and Biomolecular Engineering, University of Notre Dame

Kyle Cowdrick

Department of Chemical and Biomolecular Engineering, Harper Cancer Research Institute, University of Notre Dame

Thomas Storey

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Siyuan Zhang

Harper Cancer Research Institute, Department of Biological Sciences, University of Notre Dame

Jeremiah Zartman

Department of Chemical and Biomolecular Engineering, University of Notre Dame

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History

CURRENT STATUS: Posted

Version 1

Posted 07 Jul, 2015

No community comments so far

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Method Article

Microfluidic device design, fabrication, and testing protocols

David Hoelzle, Melinda Lake, Cody Narciso, Kyle Cowdrick, Thomas Storey, Siyuan Zhang, Jeremiah Zartman

David Hoelzle

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Corresponding Author

Melinda Lake

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Cody Narciso

Department of Chemical and Biomolecular Engineering, University of Notre Dame

Kyle Cowdrick

Department of Chemical and Biomolecular Engineering, Harper Cancer Research Institute, University of Notre Dame

Thomas Storey

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Siyuan Zhang

Harper Cancer Research Institute, Department of Biological Sciences, University of Notre Dame

Jeremiah Zartman

Department of Chemical and Biomolecular Engineering, University of Notre Dame

DOI:

10.1038/protex.2015.069

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License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Abstract

Keywords

microfluidic device, microfabrication

Introduction

Procedure

Timing

References

Acknowledgements

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History

CURRENT STATUS: Posted

Version 1

No community comments so far

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Comments: 0

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Subject Areas

Cell biology

Biological techniques

Microbiology

Learn more about our company.

This is a preprint. It has not completed peer review.

Method Article

Microfluidic device design, fabrication, and testing protocols

David Hoelzle, Melinda Lake, Cody Narciso, Kyle Cowdrick, Thomas Storey, Siyuan Zhang, Jeremiah Zartman

David Hoelzle

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Corresponding Author

Melinda Lake

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Cody Narciso

Department of Chemical and Biomolecular Engineering, University of Notre Dame

Kyle Cowdrick

Department of Chemical and Biomolecular Engineering, Harper Cancer Research Institute, University of Notre Dame

Thomas Storey

Department of Aerospace and Mechanical Engineering, Hoelzle Research Lab, University of Notre Dame

Siyuan Zhang

Harper Cancer Research Institute, Department of Biological Sciences, University of Notre Dame

Jeremiah Zartman

Department of Chemical and Biomolecular Engineering, University of Notre Dame

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This manuscript passed our Prescreen assessment

Complete author information

Appropriate declaration statements

Potential risks to human health

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