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Method Article
Paolo Cifani
Molecular Pharmacology & Chemistry Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA
Corresponding Author
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Ionization efficiency and consequently the sensitivity of mass spectrometric analyses strongly depend on the geometry of nanoelectrospray emitter used, particularly on the terminal inner diameter (ID) of the tip. This protocol has been optimized to produce emitters with a tip opening of 2-3 μm in diameter, from 50 μm ID fused silica capillaries. These emitters feature a porous silicate frit to alleviate the risk of microparticulate clogging of the narrow bore tip. As a result, they maintain stable spray performance while achieving ionization efficiencies as high as 20% without the need for specialized metal or polymer coatings. The overall time required is less than one hour.
Keywords
nanoelectrospray, mass spectrometry, emitter
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An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Paolo Cifani
Molecular Pharmacology & Chemistry Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 09 Jun, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Ionization efficiency and consequently the sensitivity of mass spectrometric analyses strongly depend on the geometry of nanoelectrospray emitter used, particularly on the terminal inner diameter (ID) of the tip. This protocol has been optimized to produce emitters with a tip opening of 2-3 μm in diameter, from 50 μm ID fused silica capillaries. These emitters feature a porous silicate frit to alleviate the risk of microparticulate clogging of the narrow bore tip. As a result, they maintain stable spray performance while achieving ionization efficiencies as high as 20% without the need for specialized metal or polymer coatings. The overall time required is less than one hour.
Figure 1
None
Loading...