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Method Article
Preparation of aggregate-free α -synuclein for in vitro aggregation study
Samir K. Maji
Indian Institute of Technology Bombay, Powai, Mumbai 400076, India
Corresponding Author
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Establishing reproducible aggregation kinetics and amyloid formation of α-synuclein (α-Syn) is of great interest for understanding Parkinson’s disease (PD) pathogenesis. α-Syn, 140 amino acid residues intrinsically disorder protein (IDP), is well known for its inconsistent aggregation behaviour in vitro. Previously different methods/conditions like orbital agitation, usage of small glass beads in plate reader assay were reported to achieve higher reproducibility. In addition to mechanical agitation, here we report the usage of aggregate free low molecular weight (LMW) solution as a starting material for monitoring aggregation pathway of α-Syn. This allowed us to obtain the reproducible fibrillation kinetics of α-Syn at a satisfactory level. This LMW could be used not only for understanding PD pathogenesis but also for screening inhibitors against α-Syn fibrillation and to study many molecular events in vitro.
Keywords
α-synuclein, low molecular weight, cut off filter, aggregates, seed
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Associated Publications
Structure based aggregation studies reveal the presence of helix-rich intermediate during α-Synuclein aggregation
by Dhiman Ghosh, Pradeep K. Singh, Shruti Sahay, +6
Scientific Reports (25 March, 2015)
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This is a preprint. It has not completed peer review.
Method Article
Preparation of aggregate-free α -synuclein for in vitro aggregation study
Samir K. Maji
Indian Institute of Technology Bombay, Powai, Mumbai 400076, India
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 18 Apr, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Establishing reproducible aggregation kinetics and amyloid formation of α-synuclein (α-Syn) is of great interest for understanding Parkinson’s disease (PD) pathogenesis. α-Syn, 140 amino acid residues intrinsically disorder protein (IDP), is well known for its inconsistent aggregation behaviour in vitro. Previously different methods/conditions like orbital agitation, usage of small glass beads in plate reader assay were reported to achieve higher reproducibility. In addition to mechanical agitation, here we report the usage of aggregate free low molecular weight (LMW) solution as a starting material for monitoring aggregation pathway of α-Syn. This allowed us to obtain the reproducible fibrillation kinetics of α-Syn at a satisfactory level. This LMW could be used not only for understanding PD pathogenesis but also for screening inhibitors against α-Syn fibrillation and to study many molecular events in vitro.
Figure 1
Figure 2
Figure 3
Associated Publications
Structure based aggregation studies reveal the presence of helix-rich intermediate during α-Synuclein aggregation
by Dhiman Ghosh, Pradeep K. Singh, Shruti Sahay, +6
Scientific Reports (25 March, 2015)