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Carol Heckman, Sucheta Kanagasundaram, Marilyn Cayer, Jong Paige
Table 1. Major protrusions are affected by temperature difference between sample and fixative. THP1 monocytic cells were adhered on fibrinogen matrix and primed with 100 ng/mL of GM-CSF overnight at 37 degrees C. Further information on the THP1 cells can be found elsewhere (Kanagasundaram, submitted for publication). The cells were fixed with 3% formaldehyde made fresh from paraformaldehyde in PBS. Sample A was equilibrated to room temperature and exposed to fixative solution that was also at room temperature. Sample B was also exposed to fixative solution at room temperature but the cells were at 37 degrees C just before fixation. In sample C, both the sample and fixative were at 37 degrees C. Actin staining was done by introducing TRITC-conjugated phalloidin. The cells were imaged by confocal microscopy. The major protrusions were measured as described in reference 3.