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Method Article
Preparation of cell-sized water-in-oil droplets for in vitro reconstitution of biological processes in cellular compartments
Makito Miyazaki
Department of Physics, Faculty of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan
Corresponding Author
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This protocol presents a method for encapsulation of purified proteins into cell-sized water-in-oil droplets surrounded by a phospholipid monolayer, which can be broadly applied to studies to reconstitute biological processes in cellular compartments.
Keywords
water-in-oil droplet, liposome, artificial cell, reconstitution, cytoskeleton, actin, myosin, contractile ring
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Associated Publications
Cell-sized spherical confinement induces the spontaneous formation of contractile actomyosin rings in vitro
by Makito Miyazaki, Masataka Chiba, Hiroki Eguchi, +2
Nature Cell Biology (23 March, 2015)
Quantitative Analysis of the Lamellarity of Giant Liposomes Prepared by the Inverted Emulsion Method
by Masataka Chiba, Makito Miyazaki, and Shin’ichi Ishiwata
Biophysical Journal (20 March, 2015)
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This is a preprint. It has not completed peer review.
Method Article
Preparation of cell-sized water-in-oil droplets for in vitro reconstitution of biological processes in cellular compartments
Makito Miyazaki
Department of Physics, Faculty of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 24 Mar, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol presents a method for encapsulation of purified proteins into cell-sized water-in-oil droplets surrounded by a phospholipid monolayer, which can be broadly applied to studies to reconstitute biological processes in cellular compartments.
Figure 1
Figure 2
Figure 3
Associated Publications
Cell-sized spherical confinement induces the spontaneous formation of contractile actomyosin rings in vitro
by Makito Miyazaki, Masataka Chiba, Hiroki Eguchi, +2
Nature Cell Biology (23 March, 2015)
Quantitative Analysis of the Lamellarity of Giant Liposomes Prepared by the Inverted Emulsion Method
by Masataka Chiba, Makito Miyazaki, and Shin’ichi Ishiwata
Biophysical Journal (20 March, 2015)