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Method Article
Makito Miyazaki
Department of Physics, Faculty of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol presents a method for encapsulation of purified proteins into cell-sized water-in-oil droplets surrounded by a phospholipid monolayer, which can be broadly applied to studies to reconstitute biological processes in cellular compartments.
Keywords
water-in-oil droplet, liposome, artificial cell, reconstitution, cytoskeleton, actin, myosin, contractile ring
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The authors declare no competing financial interests.
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Associated Publications
Cell-sized spherical confinement induces the spontaneous formation of contractile actomyosin rings in vitro
by Makito Miyazaki, Masataka Chiba, Hiroki Eguchi, +2
Nature Cell Biology (23 March, 2015)
Quantitative Analysis of the Lamellarity of Giant Liposomes Prepared by the Inverted Emulsion Method
by Masataka Chiba, Makito Miyazaki, and Shin’ichi Ishiwata
Biophysical Journal (20 March, 2015)
Method Article
Makito Miyazaki
Department of Physics, Faculty of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 24 Mar, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol presents a method for encapsulation of purified proteins into cell-sized water-in-oil droplets surrounded by a phospholipid monolayer, which can be broadly applied to studies to reconstitute biological processes in cellular compartments.
Figure 1
Figure 2
Figure 3
The authors declare no competing financial interests.
Loading...
Associated Publications
Cell-sized spherical confinement induces the spontaneous formation of contractile actomyosin rings in vitro
by Makito Miyazaki, Masataka Chiba, Hiroki Eguchi, +2
Nature Cell Biology (23 March, 2015)
Quantitative Analysis of the Lamellarity of Giant Liposomes Prepared by the Inverted Emulsion Method
by Masataka Chiba, Makito Miyazaki, and Shin’ichi Ishiwata
Biophysical Journal (20 March, 2015)