Preparation of cell-sized water-in-oil droplets for in vitro reconstitution of biological processes in cellular compartments
This protocol presents a method for encapsulation of purified proteins into cell-sized water-in-oil droplets surrounded by a phospholipid monolayer, which can be broadly applied to studies to reconstitute biological processes in cellular compartments.
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Posted 24 Mar, 2015
Preparation of cell-sized water-in-oil droplets for in vitro reconstitution of biological processes in cellular compartments
Posted 24 Mar, 2015
This protocol presents a method for encapsulation of purified proteins into cell-sized water-in-oil droplets surrounded by a phospholipid monolayer, which can be broadly applied to studies to reconstitute biological processes in cellular compartments.
Figure 1
Figure 2
Figure 3
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