MSM Trace Element (TE) solution
Trace element solution for Mineral Salts Medium (MSM, see separate protocol "http://www.nature.com/protocolexchange/protocols/3809":http://www.nature.com/protocolexchange/protocols/3809). To be added when medium is not supplied with mineral. Add 1 ml of 100x Stock TE solution into 99 ml of MSM.
Trace Element solution:
Ferric chloride hexahydrate, FeCl3 x6H2O
Copper sulfate pentahydrate, CuSO4 x5H2O
Boric acid, HBO3
Manganese sulfate hydrate, MnSO4 xH2O
Sodium molybdate dihydrate, Na2MoO4 x2H2O
Cobalt chloride hexahydrate, CoCl2 x6H2O
Zink sulfate heptahydrate, ZnSO4 x7H2O
Sodium selenate, Na2SeO4
For 100x Stock TE solution:
1100 mg/l FeCl3 x6H2O
50 mg/l CuSO4 x5H2O
200 mg/l HBO3
200 mg/l MnSO4 xH2O
80 mg/l Na2MoO4 x2H2O
60 mg/l CoCl2 x6H2O
90 mg/l ZnSO4 x7H2O
10 mg/l Na2SeO4
about 30 minutes
-TE solution should be clear and almost colorless. Strong coloration indicates too high pH.
-When encountering difficulties during dissolution of a reagent, try adding some more sulfuric acid.
Comments can take the form of short reviews, notes or questions to the author. Comments will be posted immediately. If reported by the community, we will temporarily hide the comment to determine if the comment is spam or harassment. If it is not, it will be unhidden, and it cannot be reported again. If you think a comment has been hidden or unhidden mistakenly, please contact us at [email protected].
Posted 24 Mar, 2015
© Research Square 2020