Method Article
MSM Trace Element (TE) solution
https://doi.org/10.1038/protex.2015.032
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Trace element solution for Mineral Salts Medium (MSM, see separate protocol "http://www.nature.com/protocolexchange/protocols/3809":http://www.nature.com/protocolexchange/protocols/3809). To be added when medium is not supplied with mineral. Add 1 ml of 100x Stock TE solution into 99 ml of MSM.
mineral
salt
medium
salts
cell
culture
acidophile
bioleaching
biomining
pH
acidophilic
extreme
mineral
medium
media
trace
elements
Trace Element solution:
Ferric chloride hexahydrate, FeCl3 x6H2O
Copper sulfate pentahydrate, CuSO4 x5H2O
Boric acid, HBO3
Manganese sulfate hydrate, MnSO4 xH2O
Sodium molybdate dihydrate, Na2MoO4 x2H2O
Cobalt chloride hexahydrate, CoCl2 x6H2O
Zink sulfate heptahydrate, ZnSO4 x7H2O
Sodium selenate, Na2SeO4
General:
Sulfuric acid
Sodium hydroxide
Stirring rods,
Magnetic stirrer,
Measuring colves,
pH meter
For 100x Stock TE solution:
1100 mg/l FeCl3 x6H2O
50 mg/l CuSO4 x5H2O
200 mg/l HBO3
200 mg/l MnSO4 xH2O
80 mg/l Na2MoO4 x2H2O
60 mg/l CoCl2 x6H2O
90 mg/l ZnSO4 x7H2O
10 mg/l Na2SeO4
Add the constituents into a final volume of 1 liter of water (pH pre-adjusted to around 2.5), one at a time, dissolving before adding the next.
Adjust the pH to 1.5 with sulfuric acid (and/or sodium hydroxide)
-sterile filter
about 30 minutes
-TE solution should be clear and almost colorless. Strong coloration indicates too high pH.
-When encountering difficulties during dissolution of a reagent, try adding some more sulfuric acid.
none
This protocol has been posted on Protocol Exchange, an open repository of community-contributed protocols sponsored by Nature Portfolio. These protocols are posted directly on the Protocol Exchange by authors and are made freely available to the scientific community for use and comment.
posted
You are reading this latest protocol version