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Method Article
Technique for Liquid Culture of Early Chick Embryos Suitable for Long Term Live Imaging
Cornelis Jan Weijer
Weijer Lab Dundee
Corresponding Author
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This protocol describes the culture of early stage chick embryos in liquid culture with the epiblast side up. We have designed a special culture chamber, in which the embryo is supported by a small layer of heavy silicone oil, effectively sealing of the basal side of the epiblast and forming hypoblast from the surrounding medium. Under these conditions the unincubated embryos develop to the fully extended streak stages (HH4-5) during 20 hrs of culture. These conditions are suitable for high resolution imaging with water immersion objectives in an upright or light sheet microscope.
Keywords
chick embryo, culture, imaging, gastrulation, light sheet microscopy
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Associated Publications
Myosin-II-mediated cell shape changes and cell intercalation contribute to primitive streak formation
by Emil Rozbicki, Manli Chuai, Antti I. Karjalainen, +6
Nature Cell Biology (12 October, 2015)
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This is a preprint. It has not completed peer review.
Method Article
Technique for Liquid Culture of Early Chick Embryos Suitable for Long Term Live Imaging
Cornelis Jan Weijer
Weijer Lab Dundee
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 13 Feb, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes the culture of early stage chick embryos in liquid culture with the epiblast side up. We have designed a special culture chamber, in which the embryo is supported by a small layer of heavy silicone oil, effectively sealing of the basal side of the epiblast and forming hypoblast from the surrounding medium. Under these conditions the unincubated embryos develop to the fully extended streak stages (HH4-5) during 20 hrs of culture. These conditions are suitable for high resolution imaging with water immersion objectives in an upright or light sheet microscope.
Figure 1
Figure 2
Associated Publications
Myosin-II-mediated cell shape changes and cell intercalation contribute to primitive streak formation
by Emil Rozbicki, Manli Chuai, Antti I. Karjalainen, +6
Nature Cell Biology (12 October, 2015)