Cells, reagents and consumables
MCF7, human breast cancer
LLC-PK1, pig kidney, proximal tubule
Primary cultures of superior cervical ganglion (SCG) neurons prepared from C57 or CD1 mice; Charles River, St. Constant, Quebec, Canada.
Animals were kept and handled according to the regulations of the Animal Care Committee at McGill University.
Tissue culture reagents
Growth of MCF7 and LLC-PK1 cells: Dulbecco’s Modified Eagle Medium (DMEM); Gibco supplemented with penicillin–streptomycin, 8% FBS
Growth of SCG neurons: L-15 Leibovitz medium (Sigma, L4386), supplemented with vitamins, cofactors, penicillin–streptomycin, 5% rat serum, 40 ng/ml nerve growth factor [11, 12]
Fetal Bovine Serum (FBS), Wisent or Fisher Scientific; donkey serum, Chemicon
Sodium bicarbonate, BioShop
Sodium pyruvate, Gibco
Penicillin, streptomycin, Gibco
Phosphate Buffered Saline (PBS), pH 7.4
Laminin for neuronal cultures 
Tissue culture consumables
Tissue culture flasks, Fisher Scientific
24-well dishes, Fisher Scientific
35 mm dishes, Corning
Plastic tubes, Diamed
Sterile serological pipettes, Fisher Scientific
Immunofluorescence staining reagents
Formaldehyde 37%, Fisher Scientific
Methanol, Fisher Scientific
Triton X-100, Sigma
Bovine serum albumin (BSA), BioShop
Tween 20 (Polyoxyethelenesorbitan monolaurate), Sigma
MitoTracker® CMX ROS (M7512), this compound can be fixed with formaldehyde or methanol; it remains associated with mitochondria when cells are permeabilized; Life technologies
Primary antibodies: affinity-purified antibodies against Tom70, raised in rabbits . Note: antibodies against other mitochondrial proteins may be suitable as well.
Secondary antibodies: AlexaFluor®488-conjugated anti-rabbit IgG, Jackson ImmunoResearch (711-545-152). Secondary antibodies were further purified by pre-adsorption to immobilized proteins prepared from mammalian culture cells .
4′,6-diamidino-2-phenylindole (DAPI), Sigma
Glass cover slips, circular 12 mm, size #1 (12-545-80 12CIR #1), Fisherbrand
Microscope slides, such as pre-cleaned slides (size 25×75×1 mm), Fisherbrand
Mounting Medium, for example Vectashield®, Vector Laboratories
35 mm dishes, Corning; modified according to reference  with glass coverslips, Bellco Glass Inc.