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Method Article
Maize Single Tetrad-stage Microspore Sequencing
Jianbing Yan
Yan Jianbing's Lab, Huazhong Agricultural University
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol records a method 1) to isolate single microspores from the same tetrad, 2) to lyze cell, then 3) to amplify the single cell DNA, and finaly 4) to prepare library for Illumina sequencing. The method development is based on Maize tetrad-stage microspores. Please modify Isolation steps appropriately, If your experimantal materials are not Maize.
Keywords
Single cell, Isolation, DNA amplification, Library preparing, Maize tetrad
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This is a preprint. It has not completed peer review.
Method Article
Maize Single Tetrad-stage Microspore Sequencing
Jianbing Yan
Yan Jianbing's Lab, Huazhong Agricultural University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 13 Feb, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol records a method 1) to isolate single microspores from the same tetrad, 2) to lyze cell, then 3) to amplify the single cell DNA, and finaly 4) to prepare library for Illumina sequencing. The method development is based on Maize tetrad-stage microspores. Please modify Isolation steps appropriately, If your experimantal materials are not Maize.
Figure 1
Figure 2
Figure 3