Maize Single Tetrad-stage Microspore Sequencing
This protocol records a method 1) to isolate single microspores from the same tetrad, 2) to lyze cell, then 3) to amplify the single cell DNA, and finaly 4) to prepare library for Illumina sequencing. The method development is based on Maize tetrad-stage microspores. Please modify Isolation steps appropriately, If your experimantal materials are not Maize.
Figure 1
Figure 2
Figure 3
Posted 13 Feb, 2015
Maize Single Tetrad-stage Microspore Sequencing
Posted 13 Feb, 2015
This protocol records a method 1) to isolate single microspores from the same tetrad, 2) to lyze cell, then 3) to amplify the single cell DNA, and finaly 4) to prepare library for Illumina sequencing. The method development is based on Maize tetrad-stage microspores. Please modify Isolation steps appropriately, If your experimantal materials are not Maize.
Figure 1
Figure 2
Figure 3
© Research Square 2021