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Method Article
Hai-Jun Xu
Chuan-Xi Zhang's Lab
Corresponding Author
Chuan-Xi Zhang
Chuan-Xi Zhang's Lab
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Planthoppers are members of the order Hemiptera. Some of them are among the most serious insect pests of rice in Asia. Although some species serve as model systems for ecological studies, many important biological mechanisms are still undefined at the molecular level, including wing dimorphism, virus transmission and insecticide resistance. The use of RNA interference (RNAi) could speed up the characterization of gene function in planthoppers. Here, we describe a dsRNA microinjection protocol that includes details of dsRNA synthesis, preparations of agarose plates and rearing jar, microinjection procedure, planthopper culture and RNAi verifications. We show a high efficiency of gene silencing using microinjection by RNAi in planthoppers. This protocol is simple and convenient, and should improve RNAi studies in planthoppers.
Keywords
Planthoppers, RNAi, breeding, high efficiency, protocol
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The authors declaring no competing financial interests.
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Method Article
Hai-Jun Xu
Chuan-Xi Zhang's Lab
Corresponding Author
Chuan-Xi Zhang
Chuan-Xi Zhang's Lab
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 26 Jan, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Planthoppers are members of the order Hemiptera. Some of them are among the most serious insect pests of rice in Asia. Although some species serve as model systems for ecological studies, many important biological mechanisms are still undefined at the molecular level, including wing dimorphism, virus transmission and insecticide resistance. The use of RNA interference (RNAi) could speed up the characterization of gene function in planthoppers. Here, we describe a dsRNA microinjection protocol that includes details of dsRNA synthesis, preparations of agarose plates and rearing jar, microinjection procedure, planthopper culture and RNAi verifications. We show a high efficiency of gene silencing using microinjection by RNAi in planthoppers. This protocol is simple and convenient, and should improve RNAi studies in planthoppers.
Figure 1
Figure 2
Figure 3
Figure 4
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