Isolation of extracellular vesicle RNA from cell culture supernatant
The study of extracellular RNA has been recently reported as an important mechanism of intercellular signaling. Extracellular vesicles which contain protein, mRNA, and non-coding RNA.can be isolated from cell culture supernatant. Extracellular vesicle RNA isolated from tumor cells in culture can be a useful tool for analyzing the role of either extracellular vesicles or extracellular vesicle RNA. This protocol presents a method of isolating extracellular vesicle RNA from human cholangiocarcinoma cells in culture.
Posted 26 Jan, 2015
Isolation of extracellular vesicle RNA from cell culture supernatant
Posted 26 Jan, 2015
The study of extracellular RNA has been recently reported as an important mechanism of intercellular signaling. Extracellular vesicles which contain protein, mRNA, and non-coding RNA.can be isolated from cell culture supernatant. Extracellular vesicle RNA isolated from tumor cells in culture can be a useful tool for analyzing the role of either extracellular vesicles or extracellular vesicle RNA. This protocol presents a method of isolating extracellular vesicle RNA from human cholangiocarcinoma cells in culture.
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