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Method Article
Tushar Patel
Patel Lab, Mayo Clinic
Corresponding Author
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The study of extracellular RNA has been recently reported as an important mechanism of intercellular signaling. Extracellular vesicles which contain protein, mRNA, and non-coding RNA.can be isolated from cell culture supernatant. Extracellular vesicle RNA isolated from tumor cells in culture can be a useful tool for analyzing the role of either extracellular vesicles or extracellular vesicle RNA. This protocol presents a method of isolating extracellular vesicle RNA from human cholangiocarcinoma cells in culture.
Keywords
Extracellular vesicles, Tumor Cells, Isolation, RNA
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Associated Publications
Extracellular vesicle-mediated transfer of long non-coding RNA ROR modulates chemosensitivity in human hepatocellular cancer
by Kenji Takahashi, Irene K. Yan, Takayuki Kogure, +2
Involvement of Extracellular Vesicle Long Noncoding RNA (linc-VLDLR) in Tumor Cell Responses to Chemotherapy
by K. Takahashi, I. K. Yan, J. Wood, +2
Modulation of hypoxia-signaling pathways by extracellular linc-RoR
by K. Takahashi, I. K. Yan, H. Haga, +1
Journal Of Cell Science (16 January, 2015)
Intercellular nanovesicle-mediated microRNA transfer: A mechanism of environmental modulation of hepatocellular cancer cell growth
by Takayuki Kogure, Wen-Lang Lin, Irene K. Yan, +2
Hepatology (16 January, 2015)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Tushar Patel
Patel Lab, Mayo Clinic
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 26 Jan, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The study of extracellular RNA has been recently reported as an important mechanism of intercellular signaling. Extracellular vesicles which contain protein, mRNA, and non-coding RNA.can be isolated from cell culture supernatant. Extracellular vesicle RNA isolated from tumor cells in culture can be a useful tool for analyzing the role of either extracellular vesicles or extracellular vesicle RNA. This protocol presents a method of isolating extracellular vesicle RNA from human cholangiocarcinoma cells in culture.
None
Loading...
Associated Publications
Extracellular vesicle-mediated transfer of long non-coding RNA ROR modulates chemosensitivity in human hepatocellular cancer
by Kenji Takahashi, Irene K. Yan, Takayuki Kogure, +2
Involvement of Extracellular Vesicle Long Noncoding RNA (linc-VLDLR) in Tumor Cell Responses to Chemotherapy
by K. Takahashi, I. K. Yan, J. Wood, +2
Modulation of hypoxia-signaling pathways by extracellular linc-RoR
by K. Takahashi, I. K. Yan, H. Haga, +1
Journal Of Cell Science (16 January, 2015)
Intercellular nanovesicle-mediated microRNA transfer: A mechanism of environmental modulation of hepatocellular cancer cell growth
by Takayuki Kogure, Wen-Lang Lin, Irene K. Yan, +2
Hepatology (16 January, 2015)