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Method Article
Isolation of extracellular vesicle RNA from cell culture supernatant
Tushar Patel
Patel Lab, Mayo Clinic
Corresponding Author
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The study of extracellular RNA has been recently reported as an important mechanism of intercellular signaling. Extracellular vesicles which contain protein, mRNA, and non-coding RNA.can be isolated from cell culture supernatant. Extracellular vesicle RNA isolated from tumor cells in culture can be a useful tool for analyzing the role of either extracellular vesicles or extracellular vesicle RNA. This protocol presents a method of isolating extracellular vesicle RNA from human cholangiocarcinoma cells in culture.
Keywords
Extracellular vesicles, Tumor Cells, Isolation, RNA
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Associated Publications
Extracellular vesicle-mediated transfer of long non-coding RNA ROR modulates chemosensitivity in human hepatocellular cancer
by Kenji Takahashi, Irene K. Yan, Takayuki Kogure, +2
Involvement of Extracellular Vesicle Long Noncoding RNA (linc-VLDLR) in Tumor Cell Responses to Chemotherapy
by K. Takahashi, I. K. Yan, J. Wood, +2
Modulation of hypoxia-signaling pathways by extracellular linc-RoR
by K. Takahashi, I. K. Yan, H. Haga, +1
Journal Of Cell Science (16 January, 2015)
Intercellular nanovesicle-mediated microRNA transfer: A mechanism of environmental modulation of hepatocellular cancer cell growth
by Takayuki Kogure, Wen-Lang Lin, Irene K. Yan, +2
Hepatology (16 January, 2015)
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This is a preprint. It has not completed peer review.
Method Article
Isolation of extracellular vesicle RNA from cell culture supernatant
Tushar Patel
Patel Lab, Mayo Clinic
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 26 Jan, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The study of extracellular RNA has been recently reported as an important mechanism of intercellular signaling. Extracellular vesicles which contain protein, mRNA, and non-coding RNA.can be isolated from cell culture supernatant. Extracellular vesicle RNA isolated from tumor cells in culture can be a useful tool for analyzing the role of either extracellular vesicles or extracellular vesicle RNA. This protocol presents a method of isolating extracellular vesicle RNA from human cholangiocarcinoma cells in culture.
Associated Publications
Extracellular vesicle-mediated transfer of long non-coding RNA ROR modulates chemosensitivity in human hepatocellular cancer
by Kenji Takahashi, Irene K. Yan, Takayuki Kogure, +2
Involvement of Extracellular Vesicle Long Noncoding RNA (linc-VLDLR) in Tumor Cell Responses to Chemotherapy
by K. Takahashi, I. K. Yan, J. Wood, +2
Modulation of hypoxia-signaling pathways by extracellular linc-RoR
by K. Takahashi, I. K. Yan, H. Haga, +1
Journal Of Cell Science (16 January, 2015)
Intercellular nanovesicle-mediated microRNA transfer: A mechanism of environmental modulation of hepatocellular cancer cell growth
by Takayuki Kogure, Wen-Lang Lin, Irene K. Yan, +2
Hepatology (16 January, 2015)