Method Article
Hydrodynamics-based gene transduction
https://doi.org/10.1038/nprot.2006.137
This protocol has been posted on Protocol Exchange, an open repository of community-contributed protocols sponsored by Nature Portfolio. These protocols are posted directly on the Protocol Exchange by authors and are made freely available to the scientific community for use and comment.
posted
You are reading this latest protocol version
This highly efficient in vivo gene transduction technique for laboratory mice was performed basically as described previously (Gene Therapy. 1999 Jul; 6(7):1258-1266). Hepatocytes are most effectively transduced by tail vein injection of a large volume of DNA solution in a short time. Practice with the injection technique is necessary!!!
Reagents:
Sterile phosphate buffered saline (PBS), room temperature.
Expression vector plasmid DNA.
3ml syringe.
27-gauge needle.
Heatlamp (Technilab Instruments, Pequannock, NJ).
Mouse holder, if necessary (Mouse Tail Illuminator, Braintree Scientific Inc., MA).
Liu F, Song Y and Liu D. Gene Therapy. 1999 Jul; 6(7):1258-1266
This protocol has been posted on Protocol Exchange, an open repository of community-contributed protocols sponsored by Nature Portfolio. These protocols are posted directly on the Protocol Exchange by authors and are made freely available to the scientific community for use and comment.
posted
You are reading this latest protocol version