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Method Article
Tamoxifen Administration for Lineage Tracing Using CreERT2 mice
Andrew Vaughan
Chapman Lab, University of California - San Francisco
Corresponding Author
Alexis Brumwell
Chapman Lab, University of California - San Francisco
Corresponding Author
Harold Chapman
Chapman Lab, University of California - San Francisco
Corresponding Author
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A requirement for lineage analysis is tight temporal control of label induction. In the context of the tamoxifen-inducible cre/lox system, this means that there can be no residual tamoxifen to activate cre driven by promoters that might become upregulated over time or following injury. However, the protracted half-life of tamoxifen can allow recombination longer than generally appreciated. This protocol describes the appropriate "chase" times necessary to ensure accurate lineage analysis.
Keywords
tamoxifen, recombination, prolonged
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Associated Publications
Lineage-negative progenitors mobilize to regenerate lung epithelium after major injury
by Andrew E. Vaughan, Alexis N. Brumwell, Ying Xi, +10
Nature (30 January, 2015)
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This is a preprint. It has not completed peer review.
Method Article
Tamoxifen Administration for Lineage Tracing Using CreERT2 mice
Andrew Vaughan
Chapman Lab, University of California - San Francisco
Corresponding Author
Alexis Brumwell
Chapman Lab, University of California - San Francisco
Corresponding Author
Harold Chapman
Chapman Lab, University of California - San Francisco
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 23 Feb, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
A requirement for lineage analysis is tight temporal control of label induction. In the context of the tamoxifen-inducible cre/lox system, this means that there can be no residual tamoxifen to activate cre driven by promoters that might become upregulated over time or following injury. However, the protracted half-life of tamoxifen can allow recombination longer than generally appreciated. This protocol describes the appropriate "chase" times necessary to ensure accurate lineage analysis.
Associated Publications
Lineage-negative progenitors mobilize to regenerate lung epithelium after major injury
by Andrew E. Vaughan, Alexis N. Brumwell, Ying Xi, +10
Nature (30 January, 2015)