An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Andrew Vaughan
Chapman Lab, University of California - San Francisco
Corresponding Author
Alexis Brumwell
Chapman Lab, University of California - San Francisco
Corresponding Author
Harold Chapman
Chapman Lab, University of California - San Francisco
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
A requirement for lineage analysis is tight temporal control of label induction. In the context of the tamoxifen-inducible cre/lox system, this means that there can be no residual tamoxifen to activate cre driven by promoters that might become upregulated over time or following injury. However, the protracted half-life of tamoxifen can allow recombination longer than generally appreciated. This protocol describes the appropriate "chase" times necessary to ensure accurate lineage analysis.
Keywords
tamoxifen, recombination, prolonged
None.
Loading...
Version History
CURRENT STATUS: Posted
Version 1
Posted 23 Feb, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Associated Publications
Lineage-negative progenitors mobilize to regenerate lung epithelium after major injury
by Andrew E. Vaughan, Alexis N. Brumwell, Ying Xi, +10
Nature (30 January, 2015)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Andrew Vaughan
Chapman Lab, University of California - San Francisco
Corresponding Author
Alexis Brumwell
Chapman Lab, University of California - San Francisco
Corresponding Author
Harold Chapman
Chapman Lab, University of California - San Francisco
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 23 Feb, 2015
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
A requirement for lineage analysis is tight temporal control of label induction. In the context of the tamoxifen-inducible cre/lox system, this means that there can be no residual tamoxifen to activate cre driven by promoters that might become upregulated over time or following injury. However, the protracted half-life of tamoxifen can allow recombination longer than generally appreciated. This protocol describes the appropriate "chase" times necessary to ensure accurate lineage analysis.
None.
Loading...
Associated Publications
Lineage-negative progenitors mobilize to regenerate lung epithelium after major injury
by Andrew E. Vaughan, Alexis N. Brumwell, Ying Xi, +10
Nature (30 January, 2015)