Its 21st century scientists are attempting to provide the best lives to the society.
Medication is one of the most important aspects in our lives. Chirality in the drugs is a
complex phenomenon creating confusion in medications. The demand of chiral drugs is
increasing constantly due to different pahramaceutical activities of drugs enantiomers. One
enantiomers may be active while the other inactive, toxic or ballast; leading to various side
effects and problems . It is because of chiral nature of our biological systems. Mostly
biological reactions are stereo-selective because of different enantioselective distribution
rates, metabolisms, excretion and clearances of enantiomers. Due to these facts, scientists,
clinicians, industrialists, academicians and government authorities are asking data for
optically active drugs and other biological important molecules. US FDA, Health Canada,
European Committee for Proprietary Medicinal Products and Pharmaceutical and Medical
Devices Agencies of Japan, have banned the marketing of all racemic drugs [2-4].
Small peptides (monomers n < 6) are of great importance because of contribution in
various biological processes. The biological activities of small peptides include protein
synthesis, fertility, neurotransmission, inflammation process, pathogenic microorganisms
activities and other functions of human beings. These functions made peptide vital molecules
in drug development and health care [5-7]. Besides, these peptides are also being used as
biological markers in the biological systems . The small peptides are also considered as
important molecules in food and nutrition industries. For examples, aspartame, carnosine, etc.
are being prepared at industrial scale . It is important to mention here that biological
functions of peptides are stereoselective; especially related to enzymatic reactions.
In view of these facts, stereo-separation of small peptides is very important in drugs
development and health care. Stereo-separation of peptides may be achieved by capillary
electrophoresis and chromatography. Literature has many papers on stereo-separation of peptides [9-12]. Recently, Ali et al.  reviewed stereo-separations of small peptides by
capillary electrophoresis and chromatography techniques. It was observed that all these
papers contain sufficient information on stereo-separations of peptides but no one describes
the experimental procedures, methods development and optimization strategies in details,
which are urgently required at laboratory level globally. Therefore, the attempts have been
made to describe a protocol for stereo-separations of peptides by capillary electrophoresis and
chromatography. The present article describes the state-of-the-art of stereo-separations of
peptides using capillary electrophoresis, chromatography. The efforts have been made to
discuss optimization strategies and future perspectives of stereo-separations of peptides.