Enzymatic measurements for the detection of trypsin and carboxypeptidase A inhibitory activity

doi:10.1038/nprot.2007.386

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Method Article

Oscar Yanes, Josep Villanueva, Enrique Querol, Francesc Aviles

Oscar Yanes

Institut de Biotecnologia i de Biomedicina and Departament de Bioqumica, Universitat Autnoma de Barcelona, 08193 Bellaterra (Barcelona), Spain

Josep Villanueva

Protein Center and Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA.

Enrique Querol

Institut de Biotecnologia i de Biomedicina and Departament de Bioqumica, Universitat Autnoma de Barcelona, 08193 Bellaterra (Barcelona), Spain

Francesc Aviles

Institut de Biotecnologia i de Biomedicina and Departament de Bioqumica, Universitat Autnoma de Barcelona, 08193 Bellaterra (Barcelona), Spain

DOI:

10.1038/nprot.2007.386

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Associated Publications

Detection of non-covalent protein interactions by 'intensity fading' MALDI-TOF mass spectrometry: applications to proteases and protease inhibitors
by Oscar Yanes, Josep Villanueva, Enrique Querol & Francesc X Aviles
Nature (04 September, 2007)

An open repository of community-contributed protocols sponsored by Nature Research.

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Method Article

Oscar Yanes, Josep Villanueva, Enrique Querol, Francesc Aviles

Oscar Yanes

Institut de Biotecnologia i de Biomedicina and Departament de Bioqumica, Universitat Autnoma de Barcelona, 08193 Bellaterra (Barcelona), Spain

Josep Villanueva

Protein Center and Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA.

Enrique Querol

Institut de Biotecnologia i de Biomedicina and Departament de Bioqumica, Universitat Autnoma de Barcelona, 08193 Bellaterra (Barcelona), Spain

Francesc Aviles

Institut de Biotecnologia i de Biomedicina and Departament de Bioqumica, Universitat Autnoma de Barcelona, 08193 Bellaterra (Barcelona), Spain

Version History

CURRENT STATUS: Posted

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Posted 04 Sep, 2007

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Subject Areas

Biological techniques

Structural biology

Chemical biology

Drug discovery

Biochemistry

Computational biology and bioinformatics

DOI:

10.1038/nprot.2007.386

Download PDF

The full text of this article is available to read as a PDF.

Associated Publications

Detection of non-covalent protein interactions by 'intensity fading' MALDI-TOF mass spectrometry: applications to proteases and protease inhibitors
by Oscar Yanes, Josep Villanueva, Enrique Querol & Francesc X Aviles
Nature (04 September, 2007)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Oscar Yanes, Josep Villanueva, Enrique Querol, Francesc Aviles

Oscar Yanes

Institut de Biotecnologia i de Biomedicina and Departament de Bioqumica, Universitat Autnoma de Barcelona, 08193 Bellaterra (Barcelona), Spain

Josep Villanueva

Protein Center and Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA.

Enrique Querol

Institut de Biotecnologia i de Biomedicina and Departament de Bioqumica, Universitat Autnoma de Barcelona, 08193 Bellaterra (Barcelona), Spain

Francesc Aviles

Institut de Biotecnologia i de Biomedicina and Departament de Bioqumica, Universitat Autnoma de Barcelona, 08193 Bellaterra (Barcelona), Spain

DOI:

10.1038/nprot.2007.386

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Biological techniques

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Biochemistry

Computational biology and bioinformatics

Associated Publications

Detection of non-covalent protein interactions by 'intensity fading' MALDI-TOF mass spectrometry: applications to proteases and protease inhibitors

by Oscar Yanes, Josep Villanueva, Enrique Querol & Francesc X Aviles

Nature (04 September, 2007)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Oscar Yanes, Josep Villanueva, Enrique Querol, Francesc Aviles

Oscar Yanes

Institut de Biotecnologia i de Biomedicina and Departament de Bioqumica, Universitat Autnoma de Barcelona, 08193 Bellaterra (Barcelona), Spain

Josep Villanueva

Protein Center and Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA.

Enrique Querol

Institut de Biotecnologia i de Biomedicina and Departament de Bioqumica, Universitat Autnoma de Barcelona, 08193 Bellaterra (Barcelona), Spain

Francesc Aviles

Institut de Biotecnologia i de Biomedicina and Departament de Bioqumica, Universitat Autnoma de Barcelona, 08193 Bellaterra (Barcelona), Spain

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DOI:

10.1038/nprot.2007.386

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Associated Publications

Detection of non-covalent protein interactions by 'intensity fading' MALDI-TOF mass spectrometry: applications to proteases and protease inhibitors