Bt-CoV HKU5 PCR/Sequencing Primers

doi:10.1038/protex.2014.023

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Method Article

Rachel Graham

ORFeome

Corresponding Author

DOI:

10.1038/protex.2014.023

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This protocol details the primers and conditions used for forward and reverse PCR amplification and sequencing of Bt-CoV HKU5 genomes.

Keywords

coronavirus, HKU5, genetic analysis, sequencing

None.

This is a list of supplementary files associated with this preprint. Click to download.

supplement0.docx
Table 1 Bt-CoV HKU5 Sequencing Primers
supplement0.doc
Table 3 Bt-CoV HKU5 Amplicon Primer Sets
supplement0.doc
Table 2 SARS-CoV Sequencing Primers

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Subject Areas

Genetics

Microbiology

Associated Publications

A Mouse Model for Betacoronavirus Subgroup 2c Using a Bat Coronavirus Strain HKU5 Variant
by S. Agnihothram, B. L. Yount, E. F. Donaldson, +16

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Rachel Graham

ORFeome

Corresponding Author

Version History

CURRENT STATUS: Posted

Version 1

Posted 10 Jul, 2014

No community comments so far

MetricsComments: 0HTML Views: ...

Subject Areas

Genetics

Microbiology

DOI:

10.1038/protex.2014.023

Download PDF

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

This protocol details the primers and conditions used for forward and reverse PCR amplification and sequencing of Bt-CoV HKU5 genomes.

None.

This is a list of supplementary files associated with this preprint. Click to download.

supplement0.docx
Table 1 Bt-CoV HKU5 Sequencing Primers
supplement0.doc
Table 3 Bt-CoV HKU5 Amplicon Primer Sets
supplement0.doc
Table 2 SARS-CoV Sequencing Primers

Associated Publications

A Mouse Model for Betacoronavirus Subgroup 2c Using a Bat Coronavirus Strain HKU5 Variant
by S. Agnihothram, B. L. Yount, E. F. Donaldson, +16

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Rachel Graham

Rachel Graham

ORFeome

Corresponding Author

DOI:

10.1038/protex.2014.023

Download PDF

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Abstract

Keywords

coronavirus, HKU5, genetic analysis, sequencing

Introduction

Reagents

Equipment

Procedure

Timing

Anticipated Results

Competing Interests

Supplementary Files

Comments

Version History

CURRENT STATUS: Posted

Version 1

No community comments so far

Metrics

Comments: 0

HTML Views: ...

Subject Areas

Genetics

Microbiology

Associated Publications

A Mouse Model for Betacoronavirus Subgroup 2c Using a Bat Coronavirus Strain HKU5 Variant

by S. Agnihothram, B. L. Yount, E. F. Donaldson, +16

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Rachel Graham

Rachel Graham

ORFeome

Corresponding Author

Badges

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Version History

CURRENT STATUS: Posted

Version 1

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Metrics

Comments: 0

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Subject Areas

Genetics

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DOI & PDF

DOI:

10.1038/protex.2014.023

Download PDF

License

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Declarations

Abstract

Introduction

Reagents

Equipment

Procedure

Timing

Anticipated Results

Competing Interests

Supplementary Files

Comments

Related Articles

Associated Publications

A Mouse Model for Betacoronavirus Subgroup 2c Using a Bat Coronavirus Strain HKU5 Variant

by S. Agnihothram, B. L. Yount, E. F. Donaldson, +16