Quantitative Procedure to Analyze Nuclear β-Catenin Using Immunofluorescence Tissue Staining
The analysis of the amounts of several proteins, which are found in different sub-cellular compartments, by immunofluorescence can be affected by artifacts that need to be detected and, if possible, corrected. In the case of nuclear β-catenin, its detection is usually affected by the signal obtained from the plasma membrane of the cells (which contain the most abundant pool of the beta-catenin). Here we describe an improved method for relative quantification of nuclear β-catenin amounts in immunofluorescent staining of human colon carcinoma FFPE samples.
Posted 05 Jun, 2014
Quantitative Procedure to Analyze Nuclear β-Catenin Using Immunofluorescence Tissue Staining
Posted 05 Jun, 2014
The analysis of the amounts of several proteins, which are found in different sub-cellular compartments, by immunofluorescence can be affected by artifacts that need to be detected and, if possible, corrected. In the case of nuclear β-catenin, its detection is usually affected by the signal obtained from the plasma membrane of the cells (which contain the most abundant pool of the beta-catenin). Here we describe an improved method for relative quantification of nuclear β-catenin amounts in immunofluorescent staining of human colon carcinoma FFPE samples.
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