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Method Article
Oriol Arqués
H.G. Palmer's Lab, Stem Cells and Cancer
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The analysis of the amounts of several proteins, which are found in different sub-cellular compartments, by immunofluorescence can be affected by artifacts that need to be detected and, if possible, corrected. In the case of nuclear β-catenin, its detection is usually affected by the signal obtained from the plasma membrane of the cells (which contain the most abundant pool of the beta-catenin). Here we describe an improved method for relative quantification of nuclear β-catenin amounts in immunofluorescent staining of human colon carcinoma FFPE samples.
Keywords
β-Catenin, alpha-catenin, confocal microscopy, ImageJ
The authors declare that they have no competing financial interests.
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An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Oriol Arqués
H.G. Palmer's Lab, Stem Cells and Cancer
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 05 Jun, 2014
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The analysis of the amounts of several proteins, which are found in different sub-cellular compartments, by immunofluorescence can be affected by artifacts that need to be detected and, if possible, corrected. In the case of nuclear β-catenin, its detection is usually affected by the signal obtained from the plasma membrane of the cells (which contain the most abundant pool of the beta-catenin). Here we describe an improved method for relative quantification of nuclear β-catenin amounts in immunofluorescent staining of human colon carcinoma FFPE samples.
The authors declare that they have no competing financial interests.
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