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Method Article
Quantitative Procedure to Analyze Nuclear β-Catenin Using Immunofluorescence Tissue Staining
Oriol Arqués
H.G. Palmer's Lab, Stem Cells and Cancer
Corresponding Author
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This work is licensed under a CC BY-NC 3.0 License. Read Full License
The analysis of the amounts of several proteins, which are found in different sub-cellular compartments, by immunofluorescence can be affected by artifacts that need to be detected and, if possible, corrected. In the case of nuclear β-catenin, its detection is usually affected by the signal obtained from the plasma membrane of the cells (which contain the most abundant pool of the beta-catenin). Here we describe an improved method for relative quantification of nuclear β-catenin amounts in immunofluorescent staining of human colon carcinoma FFPE samples.
Keywords
β-Catenin, alpha-catenin, confocal microscopy, ImageJ
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This is a preprint. It has not completed peer review.
Method Article
Quantitative Procedure to Analyze Nuclear β-Catenin Using Immunofluorescence Tissue Staining
Oriol Arqués
H.G. Palmer's Lab, Stem Cells and Cancer
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 05 Jun, 2014
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The analysis of the amounts of several proteins, which are found in different sub-cellular compartments, by immunofluorescence can be affected by artifacts that need to be detected and, if possible, corrected. In the case of nuclear β-catenin, its detection is usually affected by the signal obtained from the plasma membrane of the cells (which contain the most abundant pool of the beta-catenin). Here we describe an improved method for relative quantification of nuclear β-catenin amounts in immunofluorescent staining of human colon carcinoma FFPE samples.