・ 500 mM Tris-HCl (pH 8.0) (Sigma-Aldrich Corp., St. Louis, MO, USA; cat. no. 93349)
・ 0.5 mM Ethylenediaminetetraacetic acid (ETDA; Sigma-Aldrich Japan, Tokyo, Japan; cat. no. E9884)
・ 5.0 mM Dithiothreitol (DDT; Sigma-Aldrich Japan; cat. no. D0632)
・ 50% Glycerol (Sigma-Aldrich Japan; cat. no. G5516)
・ Core histones (Upstates Biotech. Co.; Charlottesville, VA, USA; cat. no. 13-107)
・ [3H]Acetyl-CoA (4.3 Ci/mmol; GE Healthcare Bioscience Co., Tokyo, Japan)
・ 0.5 mM Acetyl-CoA trilithium salt (Wako Pure Chemical Industries Ltd., Osaka, Japan; cat no. 018-10811)
・ P-81 Phosphocellulose filter paper (Upstate Biotech. Co.; cat. no. 20-134)
・ 50 mM Na2HPO4・12H2O (pH 9.0) (Wako Pure Chemical Industries Ltd.; cat. no. 194-02835)
・ Acetone (Wako Pure Chemical Industries Ltd.; cat. no. D18-01146)
・ 5x Reaction buffer; 500 mM Tris-HCl (pH 8.0), 0.5 mM EDTA, 5.0 mM DDT, 50% glycerol
・ [3H]Acetyl-CoA mix; [3H]acetyl-CoA (4.3 Ci/mmol), non-radiolabeled 0.5 mM acetyl-CoA trilithium salt and distilled water (ddH2O) combined at 1:1:3 (v/v)
・ Jun dimerization protein 2 (JDP2) purified as glutathione S-transferase-JDP2 (GST-JDP2), as described elsewhere2,3
・ p300 purified as (His)6-tagged-p300, as described elsewhere4