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Method Article
Pradip Nahar
Nahar's Lab
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
In this protocol, we report spectrophotometer-free image-based miniaturized ELISA on an activated polypropylene microtest plate (APPµTP) which is precise, reliable, rapid, and sensitive. Activated test zone in APPµTP binds a capture biomolecule through covalent linkage thereby, eliminating non-specific binding often prevalent in absorption based techniques. Efficacy of APPµTP is demonstrated by detecting human immunoglobulin G (IgG), human immunoglobulin E (IgE) and Aspergillus fumigatus antibody in patient's sera. Detection is done by taking the image of the assay solution by a desktop scanner and analyzing the color of the image. Human IgE quantification by color saturation in the image-based assay shows excellent correlation with absorbance-based assay. APPµTP can quantify an analyte as precisely as in microtiter plate and could be a good substitute for conventional immunoassay procedure widely used in clinical and research laboratories.
Keywords
Image-based ELISA Color saturation APPµTP Activated polypropylene microtest plate Clinical diagnosis Low cost assay Miniaturized assay
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Posted 31 Dec, 2013
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Subject Areas
Biological techniques
Associated Publications
Image-based ELISA on an activated polypropylene microtest plate—A spectrophotometer-free low cost assay technique
by Shahila Parween and Pradip Nahar
Biosensors And Bioelectronics (27 December, 2013)
Identification of Novel Adhesins of M. tuberculosis H37Rv Using Integrated Approach of Multiple Computational Algorithms and Experimental Analysis
by Sanjiv Kumar, Bhanwar Lal Puniya, Shahila Parween, +3
PLoS ONE (27 December, 2013)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Pradip Nahar
Nahar's Lab
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 31 Dec, 2013
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
In this protocol, we report spectrophotometer-free image-based miniaturized ELISA on an activated polypropylene microtest plate (APPµTP) which is precise, reliable, rapid, and sensitive. Activated test zone in APPµTP binds a capture biomolecule through covalent linkage thereby, eliminating non-specific binding often prevalent in absorption based techniques. Efficacy of APPµTP is demonstrated by detecting human immunoglobulin G (IgG), human immunoglobulin E (IgE) and Aspergillus fumigatus antibody in patient's sera. Detection is done by taking the image of the assay solution by a desktop scanner and analyzing the color of the image. Human IgE quantification by color saturation in the image-based assay shows excellent correlation with absorbance-based assay. APPµTP can quantify an analyte as precisely as in microtiter plate and could be a good substitute for conventional immunoassay procedure widely used in clinical and research laboratories.
Figure 1
Figure 2
none
Loading...
Associated Publications
Image-based ELISA on an activated polypropylene microtest plate—A spectrophotometer-free low cost assay technique
by Shahila Parween and Pradip Nahar
Biosensors And Bioelectronics (27 December, 2013)
Identification of Novel Adhesins of M. tuberculosis H37Rv Using Integrated Approach of Multiple Computational Algorithms and Experimental Analysis
by Sanjiv Kumar, Bhanwar Lal Puniya, Shahila Parween, +3
PLoS ONE (27 December, 2013)