In the present protocol we describe a novel TLC procedure that can sufficiently reduce the time required to isolate Histamine from biological sample like chicken meat (10g, dried basis), 96 minutes. Furthermore, Histamine isolation on TLC was best achieved when chicken meat sample was stirred at full speed (1600 RPM) on heated magnetic stirrer plate. This helps in liberating tissues bound histamine using a low temperature range, 80C0. Beyond this temperature however, viscosity of the chicken meat occurs that makes trouble in filtration of the sample. Whatman filter paper (No.1) is sufficient to filter the samples in 7.0 minutes. However, vacuum filtration or squeezing the sample contained filter paper may further reduce the time. Using TLC running buffer, methyl alcohol: ammonia (20:1) is not sufficient to rapidly mobilize TLC spots upward, instead methyl alcohol: ammonia (30:1.5) is reasonable to ablate time. TLC method is sensitive in this case and can detect histamine even other biogenic amines of biological importance, food etc. Overall, TLC procedure is satisfactory, sensitive, rapid, and above all reproducible; therefore, TLC is a good option than expensive protocol like HPLC method.