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Method Article
PCR_Sequencing for genotyping SNPs PF3D7_1343700 Kelch protein propeller domain
Didier Menard
Malaria Molecular Epidemiology Unit
Corresponding Author
Frederic Ariey
Unité d’Immunologie Moléculaire des Parasites, Institut Pasteur, Paris, France
Corresponding Author
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This work is licensed under a CC BY-NC 3.0 License. Read Full License
This procedure is designed to genotype point mutations on chromosome 13 (PF3D7_1343700) in Kelch protein propeller domain of Plasmodium falciparum, identified in a study by Ariey et al, 2013.
Keywords
Malaria, P. falciparum, artemisinin resistance, molecular marker, K13
Figure 1
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Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.pdf
Table 4 Master Mix calculation for Nested PCR for PF3D7_1343700
- supplement0.pdf
Table 5 PCR thermocycling conditions for Nested PCR for PF3D7_1343700
- supplement0.pdf
Table 1 Primary and secondary forward (F) and reverse (R) PCR primers
- supplement0.pdf
Table 2 Master Mix calculation for Primary and Secondary PCR for PF3D7_1343700
- supplement0.pdf
Table 3 PCR thermocycling conditions for Primary PCR for PF3D7_1343700
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Associated Publications
A molecular marker of artemisinin-resistant Plasmodium falciparum malaria
by Frédéric Ariey, Benoit Witkowski, Chanaki Amaratunga, +14
Nature (18 December, 2013)
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This is a preprint. It has not completed peer review.
Method Article
PCR_Sequencing for genotyping SNPs PF3D7_1343700 Kelch protein propeller domain
Didier Menard
Malaria Molecular Epidemiology Unit
Corresponding Author
Frederic Ariey
Unité d’Immunologie Moléculaire des Parasites, Institut Pasteur, Paris, France
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 19 Dec, 2013
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This procedure is designed to genotype point mutations on chromosome 13 (PF3D7_1343700) in Kelch protein propeller domain of Plasmodium falciparum, identified in a study by Ariey et al, 2013.
Figure 1
Figure 2
Figure 3
Figure 4
Associated Publications
A molecular marker of artemisinin-resistant Plasmodium falciparum malaria
by Frédéric Ariey, Benoit Witkowski, Chanaki Amaratunga, +14
Nature (18 December, 2013)