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Method Article
Pradip Nahar
Nahar's Lab
Corresponding Author
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In this protocol, we describe a unique ELISA method for the detection of an antigen or antibody, in which ELISA steps are carried out by pressure incubation instead of conventional thermal incubation. Pressure-mediated ELISA (PELISA), carried out in 1 h shows more than a 2-fold increase in absorbance value than the control experiment carried out at the same time and temperature without applying pressure. Estimation of analyte by the 1-h PELISA method gives similar absorbance value to that obtained by 3-h heat-mediated ELISA (HELISA) on an activated surface. As PELISA is sensitive, specific, and reproducible, it could be an excellent alternative to HELISA or conventional ELISA procedure.
Keywords
Pressure-mediated ELISA PELISA Enzyme-linked immunosorbent assay 1-flouro-2-nitro-4-azidobenzene Activated microtiter plate covalent immobilization
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Biological techniques
Associated Publications
Pressure: a novel tool for enzyme-linked immunosorbent assay procedure
by Dileep Kannoujia and Pradip Nahar
BioTechniques (10 December, 2013)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Pradip Nahar
Nahar's Lab
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 10 Dec, 2013
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
In this protocol, we describe a unique ELISA method for the detection of an antigen or antibody, in which ELISA steps are carried out by pressure incubation instead of conventional thermal incubation. Pressure-mediated ELISA (PELISA), carried out in 1 h shows more than a 2-fold increase in absorbance value than the control experiment carried out at the same time and temperature without applying pressure. Estimation of analyte by the 1-h PELISA method gives similar absorbance value to that obtained by 3-h heat-mediated ELISA (HELISA) on an activated surface. As PELISA is sensitive, specific, and reproducible, it could be an excellent alternative to HELISA or conventional ELISA procedure.
Figure 1
none
Loading...
Associated Publications
Pressure: a novel tool for enzyme-linked immunosorbent assay procedure
by Dileep Kannoujia and Pradip Nahar
BioTechniques (10 December, 2013)