A novel enzyme-linked immunosorbent assay procedure in one hour by pressure incubation
In this protocol, we describe a unique ELISA method for the detection of an antigen or antibody, in which ELISA steps are carried out by pressure incubation instead of conventional thermal incubation. Pressure-mediated ELISA (PELISA), carried out in 1 h shows more than a 2-fold increase in absorbance value than the control experiment carried out at the same time and temperature without applying pressure. Estimation of analyte by the 1-h PELISA method gives similar absorbance value to that obtained by 3-h heat-mediated ELISA (HELISA) on an activated surface. As PELISA is sensitive, specific, and reproducible, it could be an excellent alternative to HELISA or conventional ELISA procedure.
Figure 1
Posted 10 Dec, 2013
A novel enzyme-linked immunosorbent assay procedure in one hour by pressure incubation
Posted 10 Dec, 2013
In this protocol, we describe a unique ELISA method for the detection of an antigen or antibody, in which ELISA steps are carried out by pressure incubation instead of conventional thermal incubation. Pressure-mediated ELISA (PELISA), carried out in 1 h shows more than a 2-fold increase in absorbance value than the control experiment carried out at the same time and temperature without applying pressure. Estimation of analyte by the 1-h PELISA method gives similar absorbance value to that obtained by 3-h heat-mediated ELISA (HELISA) on an activated surface. As PELISA is sensitive, specific, and reproducible, it could be an excellent alternative to HELISA or conventional ELISA procedure.
Figure 1
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