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Method Article
A novel enzyme-linked immunosorbent assay procedure in one hour by pressure incubation
Pradip Nahar
Nahar's Lab
Corresponding Author
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In this protocol, we describe a unique ELISA method for the detection of an antigen or antibody, in which ELISA steps are carried out by pressure incubation instead of conventional thermal incubation. Pressure-mediated ELISA (PELISA), carried out in 1 h shows more than a 2-fold increase in absorbance value than the control experiment carried out at the same time and temperature without applying pressure. Estimation of analyte by the 1-h PELISA method gives similar absorbance value to that obtained by 3-h heat-mediated ELISA (HELISA) on an activated surface. As PELISA is sensitive, specific, and reproducible, it could be an excellent alternative to HELISA or conventional ELISA procedure.
Keywords
Pressure-mediated ELISA PELISA Enzyme-linked immunosorbent assay 1-flouro-2-nitro-4-azidobenzene Activated microtiter plate covalent immobilization
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Associated Publications
Pressure: a novel tool for enzyme-linked immunosorbent assay procedure
by Dileep Kannoujia and Pradip Nahar
BioTechniques (10 December, 2013)
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This is a preprint. It has not completed peer review.
Method Article
A novel enzyme-linked immunosorbent assay procedure in one hour by pressure incubation
Pradip Nahar
Nahar's Lab
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 10 Dec, 2013
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
In this protocol, we describe a unique ELISA method for the detection of an antigen or antibody, in which ELISA steps are carried out by pressure incubation instead of conventional thermal incubation. Pressure-mediated ELISA (PELISA), carried out in 1 h shows more than a 2-fold increase in absorbance value than the control experiment carried out at the same time and temperature without applying pressure. Estimation of analyte by the 1-h PELISA method gives similar absorbance value to that obtained by 3-h heat-mediated ELISA (HELISA) on an activated surface. As PELISA is sensitive, specific, and reproducible, it could be an excellent alternative to HELISA or conventional ELISA procedure.
Figure 1
Associated Publications
Pressure: a novel tool for enzyme-linked immunosorbent assay procedure
by Dileep Kannoujia and Pradip Nahar
BioTechniques (10 December, 2013)