We describe a novel holidic medium for Drosophila melanogaster that has been optimised for adult fitness traits. Development is also possible on this medium, albeit at a reduced rate.
On this medium, adult egg laying, lifespan and activity patterns are no different from those of flies on a sugar yeast diet.
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This is a list of supplementary files associated with this preprint. Click to download.
Table 4 Stock solutions part 3 (added after autoclaving) These are amino acid stock solutions. Note, there are two tables here, each containing all 20 amino acids, but in differet ratios. The Yaa ratio is that most similar to what is found in yeast.
Table 5 example working recipe These recipes describe the ingredient list and quantities required to make up holidic media for HUNTaa and Yaa amino acid mixtures. Note, there are three recipes for each corresponding to different final amino acid levels in the media. Modifying these levels will alter adult fitness measures.
Table 1 Ordering list for holidic diet components A list of holidic medium components with order numbers. These are supplied in an attempt to standardise how people make the media and enhance replication of data between laboratories and trials.
Table 2 Stock solutions part 1 (those added before autoclaving) These stock solutions are to be made in advance of medium preparation. This batch are added to the medium before autoclaving.
Table 3 Stock solutions part 2 (added after autoclaving) These tables contain the stock solutions to be added after autoclaving. In most cases they are filter sterilised using a syringe-mounted filter. They are added after autoclaving to avoid nutritional loss upon heating.
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Posted 18 Nov, 2013
Posted 18 Nov, 2013
We describe a novel holidic medium for Drosophila melanogaster that has been optimised for adult fitness traits. Development is also possible on this medium, albeit at a reduced rate.
On this medium, adult egg laying, lifespan and activity patterns are no different from those of flies on a sugar yeast diet.
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