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Table 4 Stock solutions part 3 (added after autoclaving) These are amino acid stock solutions.
Note, there are two tables here, each containing all 20 amino acids, but in differet ratios. The Yaa ratio is that most similar to what is found in yeast.
Table 5 example working recipe These recipes describe the ingredient list and quantities required to make up holidic media for HUNTaa and Yaa amino acid mixtures. Note, there are three recipes for each corresponding to different final amino acid levels in the media. Modifying these levels will alter adult fitness measures.
Table 1 Ordering list for holidic diet components A list of holidic medium components with order numbers. These are supplied in an attempt to standardise how people make the media and enhance replication of data between laboratories and trials.
Table 3 Stock solutions part 2 (added after autoclaving) These tables contain the stock solutions to be added after autoclaving.
In most cases they are filter sterilised using a syringe-mounted filter. They are added after autoclaving to avoid nutritional loss upon heating.