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Method Article
Matt Piper
Piper Lab
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
We describe a novel holidic medium for Drosophila melanogaster that has been optimised for adult fitness traits. Development is also possible on this medium, albeit at a reduced rate.
On this medium, adult egg laying, lifespan and activity patterns are no different from those of flies on a sugar yeast diet.
Keywords
drosophila, holidic diet, nutrition
none
This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.pdf
Table 4 Stock solutions part 3 (added after autoclaving) These are amino acid stock solutions. Note, there are two tables here, each containing all 20 amino acids, but in differet ratios. The Yaa ratio is that most similar to what is found in yeast.
- supplement0.pdf
Table 5 example working recipe These recipes describe the ingredient list and quantities required to make up holidic media for HUNTaa and Yaa amino acid mixtures. Note, there are three recipes for each corresponding to different final amino acid levels in the media. Modifying these levels will alter adult fitness measures.
- supplement0.pdf
Table 1 Ordering list for holidic diet components A list of holidic medium components with order numbers. These are supplied in an attempt to standardise how people make the media and enhance replication of data between laboratories and trials.
- supplement0.pdf
Table 2 Stock solutions part 1 (those added before autoclaving) These stock solutions are to be made in advance of medium preparation. This batch are added to the medium before autoclaving.
- supplement0.pdf
Table 3 Stock solutions part 2 (added after autoclaving) These tables contain the stock solutions to be added after autoclaving. In most cases they are filter sterilised using a syringe-mounted filter. They are added after autoclaving to avoid nutritional loss upon heating.
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Version History
CURRENT STATUS: Posted
Version 1
Posted 18 Nov, 2013
Community comments: 1
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Comments: 0
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Subject Areas
Biological techniques
Associated Publications
A holidic medium for Drosophila melanogaster
by Matthew D W Piper, Eric Blanc, Ricardo Leitão-Gonçalves, +11
Nature Methods (18 November, 2013)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Matt Piper
Piper Lab
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 18 Nov, 2013
Community comments: 1
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
We describe a novel holidic medium for Drosophila melanogaster that has been optimised for adult fitness traits. Development is also possible on this medium, albeit at a reduced rate.
On this medium, adult egg laying, lifespan and activity patterns are no different from those of flies on a sugar yeast diet.
none
This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.pdf
Table 4 Stock solutions part 3 (added after autoclaving) These are amino acid stock solutions. Note, there are two tables here, each containing all 20 amino acids, but in differet ratios. The Yaa ratio is that most similar to what is found in yeast.
- supplement0.pdf
Table 5 example working recipe These recipes describe the ingredient list and quantities required to make up holidic media for HUNTaa and Yaa amino acid mixtures. Note, there are three recipes for each corresponding to different final amino acid levels in the media. Modifying these levels will alter adult fitness measures.
- supplement0.pdf
Table 1 Ordering list for holidic diet components A list of holidic medium components with order numbers. These are supplied in an attempt to standardise how people make the media and enhance replication of data between laboratories and trials.
- supplement0.pdf
Table 2 Stock solutions part 1 (those added before autoclaving) These stock solutions are to be made in advance of medium preparation. This batch are added to the medium before autoclaving.
- supplement0.pdf
Table 3 Stock solutions part 2 (added after autoclaving) These tables contain the stock solutions to be added after autoclaving. In most cases they are filter sterilised using a syringe-mounted filter. They are added after autoclaving to avoid nutritional loss upon heating.
Loading...
Associated Publications
A holidic medium for Drosophila melanogaster
by Matthew D W Piper, Eric Blanc, Ricardo Leitão-Gonçalves, +11
Nature Methods (18 November, 2013)