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Method Article
Kenji Osafune
Osafune Lab (Kyoto University)
Corresponding Author
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The induction of kidney lineage cells from human pluripotent stem cells (hPSCs) has been previously described in some reports, but there have been no protocols so far for the robust induction of intermediate mesoderm (IM), an early embryonic germ layer that gives rise to kidneys. In the present study, we have established a robust induction protocol using combinational treatment of growth factors, which produces up to 90% IM cells expressing the IM-specific marker OSR1. This novel method thus supplies an unprecedented system to elucidate the mechanisms of IM development and potentially provide a cell source for kidney regeneration.
Keywords
Human pluripotent stem cells, Intermediate mesoderm, Kidney regeneration
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The authors declare no competing financial interests.
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Associated Publications
Monitoring and robust induction of nephrogenic intermediate mesoderm from human pluripotent stem cells
by Shin-Ichi Mae, Akemi Shono, Fumihiko Shiota, +13
Nature Communications (18 September, 2013)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Kenji Osafune
Osafune Lab (Kyoto University)
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 18 Sep, 2013
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The induction of kidney lineage cells from human pluripotent stem cells (hPSCs) has been previously described in some reports, but there have been no protocols so far for the robust induction of intermediate mesoderm (IM), an early embryonic germ layer that gives rise to kidneys. In the present study, we have established a robust induction protocol using combinational treatment of growth factors, which produces up to 90% IM cells expressing the IM-specific marker OSR1. This novel method thus supplies an unprecedented system to elucidate the mechanisms of IM development and potentially provide a cell source for kidney regeneration.
Figure 1
Figure 2
The authors declare no competing financial interests.
Loading...
Associated Publications
Monitoring and robust induction of nephrogenic intermediate mesoderm from human pluripotent stem cells
by Shin-Ichi Mae, Akemi Shono, Fumihiko Shiota, +13
Nature Communications (18 September, 2013)