Long-term co-culture of primary hepatocytes and GFP-HepaRG cells for investigations on Plasmodium hypnozoites
The dormant liver stages, hypnozoites, of some malaria parasite species were not amenable to in vitro studies because primary hepatocyte cultures could not be maintained beyond 10-14 days. In this protocol we describe a co-culture system where primary simian hepatocyte could be maintained for a month or more through co-cultivation with an immortalised liver derived cell line, Hepa-RG. The hepatocytes can be infected with Plasmodium sporozoites and the persisting hypnozoites can then observed and investigated
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Posted 11 Feb, 2014
Long-term co-culture of primary hepatocytes and GFP-HepaRG cells for investigations on Plasmodium hypnozoites
Posted 11 Feb, 2014
The dormant liver stages, hypnozoites, of some malaria parasite species were not amenable to in vitro studies because primary hepatocyte cultures could not be maintained beyond 10-14 days. In this protocol we describe a co-culture system where primary simian hepatocyte could be maintained for a month or more through co-cultivation with an immortalised liver derived cell line, Hepa-RG. The hepatocytes can be infected with Plasmodium sporozoites and the persisting hypnozoites can then observed and investigated
Figure 1
Figure 2
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