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Method Article
Long-term co-culture of primary hepatocytes and GFP-HepaRG cells for investigations on Plasmodium hypnozoites
Georges Snounou
Snounou-Mazier Lab, INSERM-UPMC
Corresponding Author
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The dormant liver stages, hypnozoites, of some malaria parasite species were not amenable to in vitro studies because primary hepatocyte cultures could not be maintained beyond 10-14 days. In this protocol we describe a co-culture system where primary simian hepatocyte could be maintained for a month or more through co-cultivation with an immortalised liver derived cell line, Hepa-RG. The hepatocytes can be infected with Plasmodium sporozoites and the persisting hypnozoites can then observed and investigated
Keywords
Cultivation, Hepatocyte, Hypnozoite, Malaria, Plasmodium
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Associated Publications
Persistence and activation of malaria hypnozoites in long-term primary hepatocyte cultures
by Laurent Dembélé, Jean-François Franetich, Audrey Lorthiois, +10
Nature Medicine (11 August, 2013)
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This is a preprint. It has not completed peer review.
Method Article
Long-term co-culture of primary hepatocytes and GFP-HepaRG cells for investigations on Plasmodium hypnozoites
Georges Snounou
Snounou-Mazier Lab, INSERM-UPMC
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 11 Feb, 2014
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The dormant liver stages, hypnozoites, of some malaria parasite species were not amenable to in vitro studies because primary hepatocyte cultures could not be maintained beyond 10-14 days. In this protocol we describe a co-culture system where primary simian hepatocyte could be maintained for a month or more through co-cultivation with an immortalised liver derived cell line, Hepa-RG. The hepatocytes can be infected with Plasmodium sporozoites and the persisting hypnozoites can then observed and investigated
Figure 1
Figure 2
Associated Publications
Persistence and activation of malaria hypnozoites in long-term primary hepatocyte cultures
by Laurent Dembélé, Jean-François Franetich, Audrey Lorthiois, +10
Nature Medicine (11 August, 2013)