Membrane remodeling is an important process implicated in several cellular functions including compartmentalization and membrane and protein trafficking. Amphiphysin 2 (BIN1) and dynamin 2 (DNM2) are membrane tubulating proteins, that are mutated in recessive and dominant centronuclear myopathy, respectively (refs 1-4). The overexpression of a muscle isoform of BIN1 (iso 8) in COS-1 cells provokes a striking rearrangement of membranes into long tubules. The large GTPase DNM2 is recruited to these membrane tubules through interaction between its proline-rich domain and the SH3 domain of BIN1. This protocol allows to create and visualise BIN1-induced membrane tubules in cultured cells, and to test the recruitment of DNM2 to the tubules.