Patch-Clamp Recording from Kenyon Cells in Acutely-Isolated Bee Brain
This protocol describes a technique for making whole-cell patch-clamp recordings from mushroom body Kenyon cells (KCs) in acutely-isolated honeybee and bumblebee brain. This technique provides significant advantages over cultured neuron recordings for studying the functional properties of KCs, due for example to their intact morphology and synaptic connections. Successful patch-clamp recordings depend on the enzymatic removal of covering membranes, but are stable for up to several hours once achieved. Patch-clamp recordings from KCs in intact bee brain have been used to study the impairment of neuronal function by cholinergic pesticides, and should enable a much greater understanding of the role of KCs in sensory learning and memory.
Posted 28 Mar, 2013
Patch-Clamp Recording from Kenyon Cells in Acutely-Isolated Bee Brain
Posted 28 Mar, 2013
This protocol describes a technique for making whole-cell patch-clamp recordings from mushroom body Kenyon cells (KCs) in acutely-isolated honeybee and bumblebee brain. This technique provides significant advantages over cultured neuron recordings for studying the functional properties of KCs, due for example to their intact morphology and synaptic connections. Successful patch-clamp recordings depend on the enzymatic removal of covering membranes, but are stable for up to several hours once achieved. Patch-clamp recordings from KCs in intact bee brain have been used to study the impairment of neuronal function by cholinergic pesticides, and should enable a much greater understanding of the role of KCs in sensory learning and memory.
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