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Method Article
Chromatin Immunoprecipitation in mouse ES cells
W.W.M. Pim Pijnappel
Pijnappel/Schöler/Timmers
Corresponding Author
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This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes how the binding of transcription factors like TFIID to gene promoters in embryonic stem cells can be tested using formaldehyde cross linking, immunoprecipitation, and qPCR analysis.
Keywords
chromatin immunoprecipitation, mouse embryonic stem cells, TFIID
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Associated Publications
A central role for TFIID in the pluripotent transcription circuitry
by W. W. M. Pim Pijnappel, Daniel Esch, Marijke P. A. Baltissen, +12
Nature (13 March, 2013)
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This is a preprint. It has not completed peer review.
Method Article
Chromatin Immunoprecipitation in mouse ES cells
W.W.M. Pim Pijnappel
Pijnappel/Schöler/Timmers
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 26 Mar, 2013
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes how the binding of transcription factors like TFIID to gene promoters in embryonic stem cells can be tested using formaldehyde cross linking, immunoprecipitation, and qPCR analysis.
Associated Publications
A central role for TFIID in the pluripotent transcription circuitry
by W. W. M. Pim Pijnappel, Daniel Esch, Marijke P. A. Baltissen, +12
Nature (13 March, 2013)