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Method Article
Protocol for lentiviral knock down in mouse ES cells
W.W.M. Pim Pijnappel
Pijnappel/Schöler/Timmers
Corresponding Author
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This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes the procedure for lentiviral-mediated knock down of transcription factors like TFIID in mouse embryonic stem cells, and how this can be used to test for factors that are required to maintain the pluripotent state.
Keywords
mouse embryonic stem cells, lentiviral knock down, TFIID
yuhua fu
commented on 04 May, 2013
This protocol is very good , I will try it following this protcols.
yuhua fu
commented on 04 May, 2013
This protocol is very good , I will try it following this protcols.
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Posted 26 Mar, 2013
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Associated Publications
A central role for TFIID in the pluripotent transcription circuitry
by W. W. M. Pim Pijnappel, Daniel Esch, Marijke P. A. Baltissen, +12
Nature (13 March, 2013)
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This is a preprint. It has not completed peer review.
Method Article
Protocol for lentiviral knock down in mouse ES cells
W.W.M. Pim Pijnappel
Pijnappel/Schöler/Timmers
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 26 Mar, 2013
Community comments: 2
Metrics
Comments: 2
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes the procedure for lentiviral-mediated knock down of transcription factors like TFIID in mouse embryonic stem cells, and how this can be used to test for factors that are required to maintain the pluripotent state.
yuhua fu
commented on 04 May, 2013
This protocol is very good , I will try it following this protcols.
yuhua fu
commented on 04 May, 2013
This protocol is very good , I will try it following this protcols.
Associated Publications
A central role for TFIID in the pluripotent transcription circuitry
by W. W. M. Pim Pijnappel, Daniel Esch, Marijke P. A. Baltissen, +12
Nature (13 March, 2013)