Tissue samples for RNA analysis were obtained from brain with Laser Capture Microdissection System (Arcturus).
Method Article
Laser capture microdissection (LCM) and RNA isolation
https://doi.org/10.1038/protex.2013.027
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Tissue samples for RNA analysis were obtained from brain with Laser Capture Microdissection System (Arcturus).
Laser capture microdissection (LCM)
Tissue samples from 12 μm-thick frozen sections for RNA analysis were obtained from each hypothalamic
nucleus or 50 captured GFP-positive or -negative cells onto a single CapSure HS LCM Cap (Arcturus Engineering, Mountain View, CA) using the following parameters: spot size = 7.5 μm; power = 50–80 mW; and pulse duration = 50–1,000 ms. Total RNAs were isolated by PicoPure RNA Isolation Kit (Arcturs Engineering), and treated with DNase I (Invitrogen).
The authors declare no competing financial interests.
This protocol has been posted on Protocol Exchange, an open repository of community-contributed protocols sponsored by Nature Portfolio. These protocols are posted directly on the Protocol Exchange by authors and are made freely available to the scientific community for use and comment.
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