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Method Article
Kang Chong
Ph.D., Professor, Vice director
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Nowadays, many methods had been developed to detect the expression levels of genes and proteins, such as q-PCR, northern blot, western blot and Immunofluorescence (IF) et al. RNA in situ hybridization as a classical method for detecting the localization of mRNAs is fond of the scientists. In our studies, we developed an efficient and systematic method to examine the mRNA expression level of genes using RNA in situ in rice. Here we will describe this useful protocol in detail.
Keywords
Plant, in situ, rice, mRNA
Figure 1
The authors declare no competing financial interests.
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Associated Publications
Activation of the WUS gene induces ectopic initiation of floral meristems on mature stem surface in Arabidopsis thaliana
by Yun-Yuan Xu, Xiao-Min Wang, Jia Li, +5
Plant Molecular Biology (12 February, 2013)
The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14
by Siyi Guo, Yunyuan Xu, Huanhuan Liu, +6
Nature Communications (12 February, 2013)
Vernalization-induced flowering in wheat is mediated by a lectin-like gene VER2
by Wei-dong Yong, Yun-yuan Xu, Wen-zhong Xu, +8
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Method Article
Kang Chong
Ph.D., Professor, Vice director
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 22 May, 2013
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Nowadays, many methods had been developed to detect the expression levels of genes and proteins, such as q-PCR, northern blot, western blot and Immunofluorescence (IF) et al. RNA in situ hybridization as a classical method for detecting the localization of mRNAs is fond of the scientists. In our studies, we developed an efficient and systematic method to examine the mRNA expression level of genes using RNA in situ in rice. Here we will describe this useful protocol in detail.
Figure 1
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Associated Publications
Activation of the WUS gene induces ectopic initiation of floral meristems on mature stem surface in Arabidopsis thaliana
by Yun-Yuan Xu, Xiao-Min Wang, Jia Li, +5
Plant Molecular Biology (12 February, 2013)
The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14
by Siyi Guo, Yunyuan Xu, Huanhuan Liu, +6
Nature Communications (12 February, 2013)
Vernalization-induced flowering in wheat is mediated by a lectin-like gene VER2
by Wei-dong Yong, Yun-yuan Xu, Wen-zhong Xu, +8
Planta (12 February, 2013)